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Scientific paper - Original scientific paper
The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media
Poljoprivreda, 27 (2021), 1; 22-29. https://doi.org/10.18047/poljo.27.1.3
Bošnjak, Dejan; Marković, Monika; Agić, Dejan; Vinković, Tomislav; Tkalec Kojić, Monika; Ravnjak, Boris; Stanisavljević, Aleksandar

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

Cite this document

Bošnjak, D., Marković, M., Agić, D., Vinković, T., Tkalec Kojić, M., Ravnjak, B. & Stanisavljević, A. (2021). The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media. Poljoprivreda, 27. (1), 22-29. doi: 10.18047/poljo.27.1.3

Bošnjak, Dejan, et al. "The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media." Poljoprivreda, vol. 27, no. 1, 2021, pp. 22-29. https://doi.org/10.18047/poljo.27.1.3

Bošnjak, Dejan, Monika Marković, Dejan Agić, Tomislav Vinković, Monika Tkalec Kojić, Boris Ravnjak and Aleksandar Stanisavljević. "The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media." Poljoprivreda 27, no. 1 (2021): 22-29. https://doi.org/10.18047/poljo.27.1.3

Bošnjak, D., et al. (2021) 'The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media', Poljoprivreda, 27(1), pp. 22-29. doi: 10.18047/poljo.27.1.3

Bošnjak D, Marković M, Agić D, Vinković T, Tkalec Kojić M, Ravnjak B, and sur.. The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media. Poljoprivreda [Internet]. 2021 June 01 [cited 2024 November 22];27(1):22-29. doi: 10.18047/poljo.27.1.3

D. Bošnjak, et al., "The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media", Poljoprivreda, vol. 27, no. 1, pp. 22-29, June 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:151:847845. [Accessed: 22 November 2024]

Metadata
Title (english)The Influence of Nutrient Media Modification on the Morphological Parameters in Raspberry (Rubus idaeus L.) Micropropagation in the Liquid and Semi-solid Media
AuthorDejan Bošnjak
AuthorMonika Marković
AuthorDejan Agić
AuthorTomislav Vinković
AuthorMonika Tkalec Kojić
AuthorBoris Ravnjak
AuthorAleksandar Stanisavljević
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Fruit Growing
Abstract (english)
Within the nursery sector, a global interest in functional fruits imposes a permanent need to improve the tissue culture technology. A modern bioreactor immersion system is currently a valid solution for the micropropagation of many cultures. At the Faculty of Agrobiotechnical Sciences Osijek (FAZOS, Croatia), a research was conducted with the aim of improving the raspberry (Rubus idaeus L., cv. HimboTop®) micropropagation protocol. The treatments implied the modifications of the MS and DKW nutrient medium (DKW – standard; DKWm – substitution of FeEDTA with the FeEDDHA; MSm – macroelement modification, 3 x CaCl2, MgSO4, and KH2PO4 + FeEDDHA), differently depending on the aggregate condition (semi‐solid and liquid SETISTM). Subsequent to 15 (SETISTM) and 30 cultivation days (semi-solid medium), respectively, a morphological parameter analysis and a multiplication success evaluation were commenced. In all treatments (DKW, DKWm, and MSm), the observed parameters (shoot height, number of leaves, leaf width and length) were significantly better in an interaction with the liquid medium. Comparing the models, a liquid medium (SETISTM) resulted in a significantly higher explant multiplication (4.02) (a semi‐solid medium - 2.74). The best reproduction quality, implying a better plant vigor with a reduced vitrification, was obtained on the MSm medium in the SETISTM. A concentration optimization of individual mesocomponents (i.e., of the macroelements) and their chemical forms (FeEDDHA) may contribute to their improved adoption and consequently to the stress suppression due to an explant transplantation. Selecting the modern technical solutions (TIS bioreactors - SETISTM) and continuously improving the protocols, we may obtain the realistically sustainable models, suitable for a massive clonal reproduction.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - domestic
Publication versionPublished version
Journal titlePoljoprivreda
Numberingvol. 27, no. 1, pp. 22-29
p-ISSN1330-7142
e-ISSN1848-8080
DOIhttps://doi.org/10.18047/poljo.27.1.3
URN:NBNurn:nbn:hr:151:847845
Publication2021-06-01
Document URLhttps://hrcak.srce.hr/258150
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-05-08 11:19:31