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master's thesis
Varijabilnost Rht gena hrvatskih sorata ozime pšenice
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of agriculture, 2014. urn:nbn:hr:151:283435
Ravlić, Ivana
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

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Ravlić, I. (2014). Varijabilnost Rht gena hrvatskih sorata ozime pšenice (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:283435

Ravlić, Ivana. "Varijabilnost Rht gena hrvatskih sorata ozime pšenice." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2014. https://urn.nsk.hr/urn:nbn:hr:151:283435

Ravlić, Ivana. "Varijabilnost Rht gena hrvatskih sorata ozime pšenice." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2014. https://urn.nsk.hr/urn:nbn:hr:151:283435

Ravlić, I. (2014). 'Varijabilnost Rht gena hrvatskih sorata ozime pšenice', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 15 August 2024, https://urn.nsk.hr/urn:nbn:hr:151:283435

Ravlić I. Varijabilnost Rht gena hrvatskih sorata ozime pšenice [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2014 [cited 2024 August 15] Available at: https://urn.nsk.hr/urn:nbn:hr:151:283435

I. Ravlić, "Varijabilnost Rht gena hrvatskih sorata ozime pšenice", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:151:283435

Metadata
TitleVarijabilnost Rht gena hrvatskih sorata ozime pšenice
Title (english)Variability of Rht genes in Croatian winter wheats
AuthorIvana Ravlić
MentorSonja Marić (mentor)
Committee memberVlado Guberac
Committee memberSonja Petrović
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2014-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Cilj ovog istraživanja bio je utvrditi varijabilnost Rht gena u 20 hrvatskih i 10 stranih ozimih pšenica pomoću mikrosatelitnog markera gwm261 i specifičnih markera za Rht-B1 i Rht-D1 gene, te rezultate usporediti s visinom pšenice u poljskim pokusima. Identificirane su četiri različite alelne varijante na Xgwm261 lokusu, od 165, 174, 192 i 197 bp. Najzastupljeniji je bio alel od 192 bp, u određenim slučajevima dijagnostičnan za Rht8 gen, prisutan u 22 sorte (73,3%), odnosno 17 (85%) hrvatskih sorti. Ispitivanjem varijabilnosti na Rht-B1 i Rht-D1 lokusima, utvrđena je zastupljenost Rht-B1b i Rht-D1b alela kod 19 sorata (63,3%) odnosno 21 sorte (70%). Ovi aleli bili su prisutni u 80% hrvatskih sorata. Kombinaciju alela od 192 bp i Rht-B1b i/ili Rht-D1b alela imalo je 63,3% sorata. Gotovo sve sorte pšenice imale su očekivane visine s obzirom na prisutnost određenih alela na Xgwm261 lokusu, između 73,4 cm (Srpanjka) i 136,3 cm (U1), iako to nije bio slučaj kod prisutnosti različitih alela Rht-B1 i Rht-D1 lokusa.
Abstract (english)
The aim of this study was to determine the variability of Rht genes in 20 Croatian and 10 foreign winter wheat varieties using microsatellite marker gwm261 and specific markers for Rht-B1 and Rht-D1 genes, and to compare the results with wheat heights obtained from field trial. Four different allels were determined on Xgwm261 locus, 165, 174, 192 i 197 bp. 192 bp allel, in certain cases diagnostic for Rht8 gene, was predominant in 22 varieties (73.3%) and 17 Croatian varieties (85%), respectively. Analysis of variability on Rht-B1 and Rht-D1 loci showed presence of Rht-B1b and Rht-D1b allels in 19 varieties (63.3%) and 21 varieties (70%), respectively. These allels were present in 80% of Croatian wheat varieties. Combination of Xgwm261- 192 bp allel, and one or both Rht-B1b, Rht-D1b allel was present in 63.3% varieties. Wheat height varied from 73.4 cm (Srpanjka) to 136.3 cm (U1), and in most cases was as expected considering allel distribution on Xgwm261 locus, which was not the case for allel distribution on Rht-B1 and Rht-D1 loci.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:283435
Study programmeTitle: Plant Production; specializations in: Plant Production, Plant Nutrition and Soil Science, Plant Selection and Seed Production, Plant Protection
Course: Plant Selection and Seed Production
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka bilinogojstva (magistar/ magistra inženjer/inženjerka bilinogojstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-02-18 09:25:57