prikaz prve stranice dokumenta Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis
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Scientific paper - Original scientific paper
Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis
Croatica Chemica Acta, 84 (2011), 2; 259-268. https://doi.org/10.5562/cca1808
Špoljarić, Jasminka; Tomić, Antonija; Vukelić, Bojana; Salopek-Sondi, Branka; Agić, Dejan; Tomić, Sanja; Abramić, Marija

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

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Špoljarić, J., Tomić, A., Vukelić, B., Salopek Sondi, B., Agić, D., Tomić, S. & Abramić, M. (2011). Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis. Croatica Chemica Acta, 84. (2), 259-268. doi: 10.5562/cca1808

Špoljarić, Jasminka, et al. "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis." Croatica Chemica Acta, vol. 84, no. 2, 2011, pp. 259-268. https://doi.org/10.5562/cca1808

Špoljarić, Jasminka, Antonija Tomić, Bojana Vukelić, Branka Salopek Sondi, Dejan Agić, Sanja Tomić and Marija Abramić. "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis." Croatica Chemica Acta 84, no. 2 (2011): 259-268. https://doi.org/10.5562/cca1808

Špoljarić, J., et al. (2011) 'Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis', Croatica Chemica Acta, 84(2), pp. 259-268. doi: 10.5562/cca1808

Špoljarić J, Tomić A, Vukelić B, Salopek Sondi B, Agić D, Tomić S, and sur.. Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis. Croatica Chemica Acta [Internet]. 2011 October 03 [cited 2024 December 28];84(2):259-268. doi: 10.5562/cca1808

J. Špoljarić, et al., "Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis", Croatica Chemica Acta, vol. 84, no. 2, pp. 259-268, October 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:151:172807. [Accessed: 28 December 2024]

Metadata
Title (english)Human Dipeptidyl Peptidase III: the Role of Asn406 in Ligand Binding and Hydrolysis
AuthorJasminka Špoljarić
AuthorAntonija Tomić
AuthorBojana Vukelić
AuthorBranka Salopek-Sondi
AuthorDejan Agić
AuthorSanja Tomić
AuthorMarija Abramić
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of agroecology and Enviroment Protection)
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Agrochemistry
Abstract (english)
Human dipeptidyl peptidase III (DPP III) is a member of the zinc-metallopeptidase family M49 with a role in intracellular protein catabolism. Ligand-free crystal structure of yeast and human orthologues provided insight into the enzyme's active center and enabled some assumptions about the enzyme - substrate interactions. The molecular modeling performed for human and yeast DPP III indicated involvement of Asn406 and Asn415, respectively in ligand binding. To investigate further the role of this asparagine residue, conserved in all eukaryotic M49 peptidases, a site-directed mutagenesis of human DPP III was carried out. Replacement of Asn406 with Gln, but not with Ser, resulted in a large decrease in the enzyme's binding affinity for competitive peptide inhibitor and in catalytic efficiency. Molecular dynamics simulations performed for enzyme-substrate complexes, revealed changed coordination of the active- site zinc ion and the orientation of the catalytically important His568, in N406Q, but not in the N406S complexes.(doi: 10.5562/cca1808)
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - domestic
Publication versionPublished version
Journal titleCroatica Chemica Acta
Numberingvol. 84, no. 2, pp. 259-268
p-ISSN0011-1643
e-ISSN1334-417X
DOIhttps://doi.org/10.5562/cca1808
URN:NBNurn:nbn:hr:151:172807
Publication2011-10-03
Document URLhttps://hrcak.srce.hr/71989
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-07-04 06:20:52