Olive pomace extracts (OPE) contain various biologically active polyphenols (PPs) with numerous health benefits. However, to exert any beneficial effects, bioactive compounds should be bioavailable, and their bioavailability depends on their release from the matrix during the gastro-intestinal (GI) digestion (bioaccessibility) and later intestinal absorption (dialisibility). The aims of this research were to determine in vitro GI stability, bioaccessibility and dialysability of PPs in terms of concentration of hydroxytyrosol (HTS) and tyrosol (TS) which were obtained by HPLC-FLD method. Samples were OPE prepared without excipient (native extract, NE), and with following excipients: beta-CD (B), randomly methylated beta-CD (RAMEB), hydroxypropyl beta-CD (HPB), and gamma-CD (G). The in vitro GI digestion consisted of two sequential steps; an initial pepsin/HCl digestion to simulate gastric conditions, followed by a digestion with bile salts/pancreatin and dialysis tubing, to simulate duodenal digestion and absorption by passive diffusion. It is concluded that all samples have relatively high gastric stability (72-108%) and bioaccessibility (66- 101%). The highest dialysability (%) in terms of HTS and TS content was obtained for HPB (16 mg/mL) sample.