Nepafenac is a nonsteroidal anti-inflammatory drug and is used for prevention and treatment of pain and inflammation after the cataract surgery. It is also used as a prophylactic drug for prevention of macular oedema after the cataract surgery in patients with diabetes. In the experimental part of this thesis, a number of experiments were carried out as a part of development of a new voltametric method for the determination of nepafenac.The influence of surface modification of working glassy carbon electrode on nepafenac response was investigated and the graphene nanoplatelets and/or carbon nanofibers in solutions with chitosan or nafion were used. The selection of a pH values of Britton-Robinson buffer solution which was used as an electrolyte solution during measurements was made. The optimization of the parameters of a method for a square-wave voltammetry was one of the steps in an experimental procedure. Cyclic voltammetry has shown that the oxidation of nepafenac on modified electrode is an adsorption-controlled electrode reaction. Furthermore, adsorption of nepafenac could be used in development of a sensitive stripping voltametric method for analysis of nepafenac. Duration of accumulation and deposition potential were determined. Last but not least, after all the parameters of the new voltammetric method for determination of nepafenac were entirely defined one part of validation of the new method was carried out. In short, an experiment which had to determine the linearity range was performed. That same experiment gave a calibration curve that could be used for a quantitative determination of nepafenac.