prikaz prve stranice dokumenta Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties
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master's thesis
Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:378527
Kanski, Ines
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Kanski, I. (2015). Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:378527

Kanski, Ines. "Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:378527

Kanski, Ines. "Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:378527

Kanski, I. (2015). 'Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:378527

Kanski I. Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:163:378527

I. Kanski, "Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:378527

Metadata
TitleTesting the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties
AuthorInes Kanski
MentorOlga Gornik (mentor)
Committee memberOlga Gornik
Committee memberSandra Šupraha Goreta
Committee memberSuzana Inić
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2015-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
U okviru ovog diplomskog rada istražena je interakcija između odabranih flavonoida (eupatorin, apigenin, apigenin-7-glukozid, kempferol, naringenin, 3-O-metilkvercetin, cijanidin, genistein, luteolin-7-glukozid) sa proteinom ERp57 koji spada u skupinu disulfid-izomeraza (PDI). Za procjenu vezanja flavonoida sa proteinom ERp57 korištena je fluorescencijska spektrofotometrija i učinak gašenja fluorescencije, dok je za ispitivanje utjecaja flavonoida na vezanje ERp57-DNA korištena migracijska elektroforeza (EMSA). Također je testiran utjecaj flavonoida na aktivnost i stabilnost proteina. Apigenin i eupatorin pokazali su najmanju konstantu disocijacije, odnosno najveći afinitet za ERp57 te ih to čini najboljim molekulama za vezanje na protein ERp57. Potvrđena je važnost hidroskilne skupine za interakciju flavonoida sa proteinom.
Abstract (english)
In this thesis, interaction between a protein that belongs to the family of protein disulfide isomerases (PDI)- protein ERp57, and chosen flavonoids- eupatorin, apigenin, apigenin-7-glucoside, kaempferol, naringenin, 3-O-Methylquercetin, cyanidin, genistein and luteolin-7-glucoside was tested. Interaction between ERp57 and flavonoids was assessed using fluorescence quenching method, while electrophorectic mobility shift assay (EMSA) was used to evaluate flavonoid effect on ERp57-DNA interaction. Flavonoid influence on protein activity and protein stability was also tested. Apigenin and eupatorin showed the lowest dissociation constant and the highest binding affinity to ERp57, making them the best compounds to be used as selective inhibitors/modulators of ERp57 biological activities.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:163:378527
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-01-22 15:11:55