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master's thesis
Kvantifikacija fekalnog eozinofilnog kationskog proteina
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. urn:nbn:hr:163:596480
Ćaleta, Ivana
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Ćaleta, I. (2018). Kvantifikacija fekalnog eozinofilnog kationskog proteina (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:596480

Ćaleta, Ivana. "Kvantifikacija fekalnog eozinofilnog kationskog proteina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:596480

Ćaleta, Ivana. "Kvantifikacija fekalnog eozinofilnog kationskog proteina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:596480

Ćaleta, I. (2018). 'Kvantifikacija fekalnog eozinofilnog kationskog proteina', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 10 May 2024, https://urn.nsk.hr/urn:nbn:hr:163:596480

Ćaleta I. Kvantifikacija fekalnog eozinofilnog kationskog proteina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2018 [cited 2024 May 10] Available at: https://urn.nsk.hr/urn:nbn:hr:163:596480

I. Ćaleta, "Kvantifikacija fekalnog eozinofilnog kationskog proteina", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:163:596480

Metadata
TitleKvantifikacija fekalnog eozinofilnog kationskog proteina
Title (english)Quantification of eosinophil cationic protein in feces
AuthorIvana Ćaleta
MentorKarmela Barišić (mentor)
Committee memberKarmela Barišić (predsjednik povjerenstva)
Committee memberRoberta Petlevski (član povjerenstva)
Committee memberRenata Zrinski Topić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2018-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
ECP je bazični protein smješten u granulama eozinofilnih granulocita koji je uključen u sustav imunosnog odgovora. U stanicama domaćina broj eozinofila korelira s koncentracijama eozinofilnog kationskog proteina (ECP) koji se oslobađa njihovom aktivacijom. Karakteristika ECP-a jest citotoksičnost i sudjelovanje u različitim upalnim procesima. Intestinalna sluznica pacijenata s upalnom bolesti crijeva (IBD) karakterizirana je oštećenjem epitelnih stanica i infiltracijom različitih upalnih stanica, uključujući i ECP. Cilj ovoga diplomskoga rada bio je ispitati učinkovitost i predložiti potencijalne modifikacije metode za kvantifikaciju fekalnog ECP-a. Za bolju ekstrakciju fekalnog ECP-a korišten je ekstrakcijski pufer s CTAB-om. Ispitane su modifikacije originalnog postupka ekstrakcije ECP-a u pogledu priređivanja homogenata uzorka fecesa i trajanja inkubacije. Također je ispitana stabilnost i ponovljivost mjerenja ECP-a. Uporabom instrumenta Phadia100 provedeno je ImmunoCAP ECP mjerenje koncentracija ECP-a u uzorcima. Bolji rezultati postignuti su homogenizacijom uzoraka ultrazvučnim sonifikatorom, te sekundarnim razrjeđenjem od 5 i 10 puta. Stabilnost uzoraka osigurana je pohranom u zamrzivaču na -20°C prije analiza.
Abstract (english)
ECP is a basic protein localized in granules of the eosinophil granulocytes and is included in immune response reactions. In host cells, eosinophil levels correlate with ECP levels that are released during their activation. Characteristic of ECP is cytotoxicity and involvement in various inflammatory processes. Intestinal mucose of the patients with IBD is characterized with damaged epitelial cells and infiltration of various inflammatory cells, including eosinophils. The goal of this research was to examine efficacy and suggest potential modifications of the method for the quantification of the ECP in stool samples. For better fecal ECP extraction, buffer mixture with CTAB was used. Modifications of the original ECP extraction procedure were examined in terms of stool samples preparation and incubation time. Stability and repeatability of ECP measurement was also tested. ImmunoCAP ECP assay preformed with Phadia100 instrument was used for measuring ECP concentrations in samples. Better results were achieved by sample homogenization with ultrasonic sonificator and secundary dilution of 5 x and 10 x. Sample stability was secured by storing in the freezer at the -20°C before assays.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:596480
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-09-26 13:21:04