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master's thesis
Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. urn:nbn:hr:163:181727
Salopek, Matija
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Salopek, M. (2018). Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:181727

Salopek, Matija. "Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:181727

Salopek, Matija. "Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:181727

Salopek, M. (2018). 'Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:181727

Salopek M. Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:181727

M. Salopek, "Razvojne promjene N-glikana neuroglikoma korteksa mozga štakora", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:163:181727

Metadata
TitleRazvojne promjene N-glikana neuroglikoma korteksa mozga štakora
Title (english)N-glycan Profile Changes in Rat Brain Neuroglycome During Brain Development
AuthorMatija Salopek
MentorGordan Lauc (mentor)
Committee memberGordan Lauc (predsjednik povjerenstva)
Committee memberAna-Marija Domijan (član povjerenstva)
Committee memberToma Keser (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2018-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Analiza N-glikana tkiva mozga postaje područje sve većeg interesa glikobiologa. Poznato je da N-glikoprofil određenog tipa tkiva ovisi o interakcijama unutar kompleksne mreže čimbenika. Jedan od važnih čimbenika je vrijeme, odnosno dob jedinke. U ovom radu, nakon izolacije, pročišćavanja i fluorescentnog obilježavanja reducirajućeg kraja N-glikani korteksa mozga štakora iz 3 različite vremenske točke razvoja (24 i 48 sati postnatalne dobi, odrasla dob) analizirani su ranije razvijenom normalnofaznom kromatografskom metodom uz fluorescencijsku detekciju (HILICUPLC- FLR; Klarić i Gudelj, 2017) uz manje modifikacije postupka. Rezultati kromatografske analize podijeljeni su u 48 frakcija, a kompozicije N-glikana u svakoj frakciji određene su interpretacijom ESI(+)-MS/MS spektara. Rezultati pokazuju statistički značajne razlike odraslih i mladih jedinki u frakcijama: 5 (odrasli – dominantna kompozicija: H3N4F1, mladi – dominantna kompozicija: H3N5), 16 (odrasli: H4N4F2 i H5N4F1, mladi: H5N4F1, izostanak H4N4F2), 27 (27.1 - odrasli: H7N2Phos2, H6N4F2, mladi: H7N2Phos2; 27.2 H5N5F1S1, H5N4F2S1), 34 (odrasli: H5N4F2S2, H5N4F1S3, H5N4F1S2, H5N6F3, mladi: H5N4F1S2). Iz ovog istraživanja nije moguće utvrditi točan utjecaj određene kompozicije/strukture, no nije moguće niti isključiti postojanje modulatorne uloge pojedinih kompozicija prilikom razvoja korteksa mozga štakora.
Abstract (english)
Brain tissue N-glycans analysis is becoming a field of intensive glycobiological research. It has been shown that the N-glycoprofile of a specific tissue types results from a complex network of interactions of various factors, one of which is time – or to be precise – the age of an organism. In this paper after isolation, purification and reducing end fluorescent labeling rat brain cortex N-glycans from 3 different temporal points (24 and 48 h post natum, adult brains) were analysed using a HILICUPLC- FLR method reported earlier (Klarić i Gudelj, 2017) with minor protocol modifications. Chromatograms were divided into 48 distinct fractions and N-glycan compositions found in each fraction were elucidated using ESI(+)-MS/MS spectra. Here, we report 4 major statistical differences with possible biological implications in fractions: : 5 (adults–dominant composition(s): H3N4F1, neonates–dominant composition(s): H3N5), 16 (adults: H4N4F2 i H5N4F1, neonates: H5N4F1, absence of H4N4F2), 27 (27.1 - adults: H7N2Phos2, H6N4F2, neonates: H7N2Phos2; 27.2 H5N5F1S1, H5N4F2S1), 34 (adults: H5N4F2S2, H5N4F1S3, H5N4F1S2, H5N6F3, neonates: H5N4F1S2). Furthermore, comprehensive research is needed to help elucidate the exact impact of these structures on brain cortex development in rats and other organisms.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:181727
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-10-01 10:56:08