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master's thesis
Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. urn:nbn:hr:163:654660
Koprivec, Isabella
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Koprivec, I. (2018). Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:654660

Koprivec, Isabella. "Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:654660

Koprivec, Isabella. "Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:654660

Koprivec, I. (2018). 'Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 24 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:654660

Koprivec I. Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2018 [cited 2024 November 24] Available at: https://urn.nsk.hr/urn:nbn:hr:163:654660

I. Koprivec, "Razvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:163:654660

Metadata
TitleRazvoj metode za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi
Title (english)Development of the method for N-glycan enrichment using PGC solid phase extraction
AuthorIsabella Koprivec
MentorToma Keser (mentor)
Committee memberToma Keser (predsjednik povjerenstva)
Committee memberKristina Pavić (član povjerenstva)
Committee memberAnita Hafner (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
N-glikom predstavlja jedinstvenu poveznicu između genskih predispozicija za razvoj pojedinih bolesti i okolišnih faktora kojima je organizam izložen te je stoga moguća njegova uporaba kao biomarkera u preventivnoj i personaliziranoj medicini. Jedan je od glavnih ciljeva moderne glikobiologije razviti brze i osjetljive metode za analizu N-glikana u tragovima. Razvijenom metodom za ukoncentriravanje N-glikana PGC ekstrakcijom na čvrstoj fazi moguće je pomoću HILIC-UPLCFLR kvantitativno odrediti 5x manje koncentracije N-glikana u odnosu na prijašnju metodu. Kako bi kromatografski profil bio prihvatljiv, tijekom analize potrebno je pripaziti na kompatibilnost reagensa s mobilnom fazom za kromatografsku analizu, ionsku jakost prilikom eluacije glikana s PGC-a i udio vode u eluatu glikana. Razvijena metoda ima potencijal korištenja u istraživanjima različitih stanja i bolesti kod kojih dolazi do promjene glikozilacijskog profila, a u kojima je potrebna veća osjetljivost zbog male količine glikana u uzorku.
Abstract (english)
N-glycome represents a unique connection between the genetic predisposition for the development of certain diseases and the environmental exposure of the organism, making it a potential biomarker in preventive and personalised medicine. Consequently, the development of fast and sensitive methods for the analysis of trace N-glycans is one of the main goals in the field. By using the newly developed PGC SPE method for the enrichment of N-glycans, followed by HILICUPLC-FLR, it is possible to quantitatively measure 5x lower concentrations of N-glycans than with previous methods. During the analysis, it is important to adjust the compatibility of the reagents with the mobile phase used in chromatography, ionic strength for eluation of the N-glycans from PGC and the amount of water in the N-glycan eluate. The method can be used in research of different conditions and diseases which are known to have an altered glycosylation profile, as well as in molecular diagnostics.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:654660
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-02-18 16:24:38