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master's thesis
In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:353408
Kocić, Ida
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Kocić, I. (2019). In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:353408

Kocić, Ida. "In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:353408

Kocić, Ida. "In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:353408

Kocić, I. (2019). 'In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 24 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:353408

Kocić I. In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2024 November 24] Available at: https://urn.nsk.hr/urn:nbn:hr:163:353408

I. Kocić, "In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:353408

Metadata
TitleIn vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta
Title (english)In vitro biocompatibility study of liposomes differing in (phospho)lipid composition with the fibroblast cells
AuthorIda Kocić
MentorŽeljka Vanić (mentor)
Committee memberŽeljka Vanić (predsjednik povjerenstva)
Committee memberAnita Hafner (član povjerenstva)
Committee memberDubravka Vitali Čepo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical technology)
Zagreb
Defense date and country2019-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Svrha ovog rada bila je ispitati biokompatibilnost/citotoksičnost različitih tipova liposoma s azitromicinom na MJ90hTERT fibroblastima. Konvencionalni, deformabilni, propilenglikol i kationski liposomi s azitromicinom međusobno su se razlikovali po (fosfo)lipidnom sastavu i prisustvu suotapala (propilenglikol). Liposomi su pripravljeni tzv. film metodom, a primjenom ekstruzije postignuta je prikladna veličina liposoma za topikalnu primjenu. Sastav liposoma utjecao je na fizikalna svojstva liposoma, posebice naboj na površini i uspješnost uklapanja azitromicina. Provedena in vitro ispitivanja citotoksičnosti pokazala su biokompatibilnost svih ispitivanih liposoma s fibroblastima u koncentracijama azitromicina s kojima se postiže adekvatan antimikrobni učinak liposomskih formulacija. Usporedba različitih tipova liposoma pokazala je najbolju kompatibilnost propilenglikol liposoma, a najmanju kationskih liposoma koja je posljedica prisutnosti kationskog lipida (DODAB-a) u fosfolipidnom dvosloju.
Abstract (english)
The aim of this study was to investigate the biocompatibility/cytotoxicity of the different types of azithromycin-loaded liposomes on the MJ90hTERT fibroblasts. Conventional, deformable, propylene glycol and cationic liposomes encapsulating azithromycin, and differing in (phospho)lipid composition and the presence of co-solvent (propylene glycol), were prepared using the film hydration method. The suitable size of the liposomes for topical application was achieved by their extrusion through the membranes of 400 and 200 nm. The composition of liposomes has proven to influence the physical properties of liposomes, particularly the surface charge and the entrapment efficiency of azithromycin. All the azithromycin-loaded liposomes were biocompatible with the fibroblasts at azithromycin concentrations showing appropriate antibacterial effect. The comparison of the different types of liposomes demonstrated the highest level of compatibility with the MJ90hTERT cells for propylene glycol liposomes and the lowest for cationic liposomes, due to the presence of the cationic lipid (DODAB) in the (phospho)lipid bilayers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:353408
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-02-21 16:49:49