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master's thesis
Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:952291
Andričević, Klara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Andričević, K. (2019). Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:952291

Andričević, Klara. "Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:952291

Andričević, Klara. "Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:952291

Andričević, K. (2019). 'Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:952291

Andričević K. Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:163:952291

K. Andričević, "Učinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:952291

Metadata
TitleUčinak eteričnog ulja vrste Satureja montana L. i timola na rast i tvorbu biofilma bakterije P. aeruginosa
Title (english)Effect of Satureja montana L. essential oil and thymol on growth and biofilm production of P. aeruginosa
AuthorKlara Andričević
MentorGordana Maravić Vlahoviček (mentor)
Committee memberGordana Maravić Vlahoviček (predsjednik povjerenstva)
Committee memberBiljana Blažeković (član povjerenstva)
Committee memberDaniela Jakšić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2019-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Pseudomonas aeruginosa je sveprisutni oportunistički patogen, koji u planktonskom obliku uzrokuje akutne, a u obliku biofilma kronične, perzistentne infekcije, česte kod pacijenata s cističnom fibrozom i dijabetičkim kroničnim ranama. Višestruki mehanizmi rezistencije i brojni drugi čimbenici pridonose izrazitoj otpornosti prema djelovanju antibiotika, što kompromitira izbor učinkovite terapije. Dobro je poznato da eterična ulja i njihove komponente imaju širok spektar antimikrobnih svojstva, međutim, novija su istraživanja utvrdila da neka eterična ulja pokazuju i antibiofilm učinak. Ovo svojstvo sugerira da bi eterična ulja i njihove komponente mogle utjecati i na učinak antibiotika u eradikciji biofilma. Stoga je cilj ovog rada bio ispitati kakav učinak postiže eterično ulje vrste Satureja montana L. i timol na rast planktonskih stanica i tvorbu biofilma bakterije P. aeruginosa ATCC 27853, samostalno i u kombinaciji s tobramicinom. Učinak na rast planktonskih stanica određen je mjerenjem optičke gustoće kulture pri 570 nm. Količina nastalog biofilma određena je mjerenjem apsorbancije pri 540 nm, a konačni učinak prikazan je kao biofilm indeks. Rezultati su pokazali da su eterično ulje vrste Satureja montana L. i timol pri subinibitornim koncentracijama učinkoviti u inhibiciji rasta planktonskih stanica bakterije P. aeruginosa ATCC 27853, samostalno, i u kombinaciji s tobramicinom. S druge strane, nalazi indiciraju da bi učinak na biofilm mogao biti suprotan, promotivan i potpuno neovisan o antibakterijskom učinku. Eterično ulje vrste Satureja montana L. i timol pri subinhibitornim koncentracijama promoviraju stvaranje biofilma. Eterično ulje vrste Satureja montana L. antagonizira promotivni učinak tobramicina pri koncentraciji koja odgovara njegovoj MIK vrijednosti na prethodno stvoreni biofilm; timol pri subinhibitornim koncentracijama antagonizira učinak, ali isto tako djeluje sinergistički s tobramicinom na prethodno formirani biofilm, ovisno o koncentraciji. Ovaj rad pridonijet će naporima za pronalaskom nove i očuvanju postojeće terapije u liječenju infekcija uzrokovanih bakterijom P. aeruginosa.
Abstract (english)
Pseudomonas aeruginosa is an opportunistic and nosocomial pathogen that in planktonic form causes acute infection, and in biofilm form causes chronic, persistent infections, common in patients with cystic fibrosis and diabetic chronic wounds. Multiple resistance mechanisms and many other factors contribute to antibiotic resistance, compromising the choice of effective treatment. It has long been recognized that essential oils and their components have a wide range of antimicrobial properties. However, recent studies have shown that some essential oils may also have antibiofilm activity. It was suggested that essential oils and their components could have an effect on antibiotic-related eradication of biofilm. Therefore, the aim of this study was to investigate the effect of Satureja montana L. essential oil and thymol on the planktonic growth and biofilm of P. aeruginosa ATCC 27853, alone and in combination with tobramycin. The effect on planktonic growth was determined by measuring optical density at 570 nm. The amount of formed biofilm was determined by measuring apsorbance at 540nm and the effect on biofilm was defined as biofilm index. The results showed that Satureja montana L. essential oil and thymol, when used in subinhibitory concentrations, could effectively inhibit the planktonic growth of P. aeruginosa ATCC 27853, both alone, and in combination with tobramycin. On the other hand, the findings suggest that effect on the biofilm may be opposite and completely independent of antibacterial effect. Satureja montana L. essential oil and thymol, when used in subinhibitory concentrations, promote biofilm formation. Satureja montana L. essential oil antagonizes the promotional effect of tobramycin on preformed biofilm, when used in concentration corresponding to its MIC value. Depending on the concentration, thymol in subinhibitory concentrations either antagonizes tobramycin or synergises with it to promote biofilm survival. Results of this work will support future studies to find new and preserve the existing therapies of P. aeruginosa infections.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:952291
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-03-05 13:04:53