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master's thesis
Praćenje metabolizma anaboličko-androgenih steroida
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:974142
Đurinek, Dino
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medicinal chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Đurinek, D. (2015). Praćenje metabolizma anaboličko-androgenih steroida (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:974142

Đurinek, Dino. "Praćenje metabolizma anaboličko-androgenih steroida." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:974142

Đurinek, Dino. "Praćenje metabolizma anaboličko-androgenih steroida." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:974142

Đurinek, D. (2015). 'Praćenje metabolizma anaboličko-androgenih steroida', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:974142

Đurinek D. Praćenje metabolizma anaboličko-androgenih steroida [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:163:974142

D. Đurinek, "Praćenje metabolizma anaboličko-androgenih steroida", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:974142

Metadata
TitlePraćenje metabolizma anaboličko-androgenih steroida
Title (english)Metabolism monitoring of anabolic-androgenic steroids
AuthorDino Đurinek
MentorMirza Bojić (mentor)
Committee memberMirza Bojić
Committee memberŽeljan Maleš
Committee memberRenata Jurišić Grubešić
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medicinal chemistry)
Zagreb
Defense date and country2015-05-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
6β-hidroksilacija testosterona predstavlja marker reakciju enzima CYP3A4/5. Ova reakcija korištena je kao ogledni primjer praćenja najznačajnije skupine nedozvoljenih tvari u sportu - anaboličko-androgenih steroida. U svrhu praćenja 6β-hidroksilacije testosterona primjenjen je vezani sustav tekućinske kromatografije visoke djelotovornosti s detektorom s diodnim nizom. Za provedbu reakcije neophodan je koenzim citokroma P450 odnosno NADPH, te drugi enzimi koji sudjeluju u prijenosu elektrona kao što su NADPH reduktaza i citokrom b5. Kako su citokromi P450 vezani na membrane endoplazmatskog retikuluma i eksprimirani u jetri idealan sustav za praćenje metaboličke aktivnosti su humani jetreni mikrosomi. Rezultati analize prikazuju se u obliku ostatne aktivnosti u odnosu na pozitivnu kontrolu, a kao negativna kontrola primjenjuje se marker inhibitor CYP3A4/5 na primjer ketokonazol. HPLC spregnut s DAD predstavlja osnovni analitički sustav za praćenje anaboličkoandrogenih steroida koji se nadograđuje sa spektrometrijom masa i nadopunjuje s plinskom kromatografijom.
Abstract (english)
6β-hydoxylation of testosterone represents a marker reaction of CYP3A4/5 enzyme. This reaction was used as an example for monitoring the most significant group of prohibited substances in sports – anabolic-androgenic steroids. High prformance liquid chromatography with diode array detector was used for monitoring 6β-hydoxylation. In order to perform the reaction, it is essential to have a coenzyme of cytochrome P450 - NADPH, and other enzymes involved in electron transfer; NADPHreductase and cytochrome b5. Human liver microsomes are ideal system for monitoring the metabolic activity because cytochrom P450 enzymes are linked to membranes of endoplasmatic reticulum and are expressed in liver. The results of analysis are usually presented as residual activity in comparison to positive control. Ketoconazole, as a marker inhibitor of CYP3A4/5, was used as a negative control. High performance liquid chromatography with diode array detector represents a basic analytical system for monitoring anabolic-androgenic steroids accompanied with mass spectrometry and gas chromatography.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:974142
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2015-12-15 14:57:58