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master's thesis
Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:758402
Kraljić, Dejan
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Kraljić, D. (2015). Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:758402

Kraljić, Dejan. "Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:758402

Kraljić, Dejan. "Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:758402

Kraljić, D. (2015). 'Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:758402

Kraljić D. Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:163:758402

D. Kraljić, "Prikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:758402

Metadata
TitlePrikladnost in vitro modela rožnice za ispitivanje paracelularnog prijenosa oftalmičkog lijeka
Title (english)The suitability of in vitro corneal epithelial model for studying paracellular transport of ophthalmic drug
AuthorDejan Kraljić
MentorJasmina Lovrić (mentor)
Committee memberJasmina Lovrić
Committee memberAnita Hafner
Committee memberDubravka Vitali Čepo
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical technology)
Zagreb
Defense date and country2015-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Ovaj diplomski rad dio je projekta čiji je glavni cilj razvoj i implementacija ekonomičnog in vitro modela epitela rožnice velikog kapaciteta, s obzirom na broj ispitanih uzoraka u jedinici vremena, i zadovoljavajućeg predviđanja bioraspoloživosti lijeka u oku ljudi. Cilj ovog diplomskog rada bio je ispitati prikladnost in vitro modela epitela rožnice za ispitivanje paracelularnog prijenosa. U tu svrhu bilo je potrebno ispitati učinak pospješivača apsorpcije, koji ostvaruju svoj učinak otvarajući čvrste veze, na permeabilnost obilježivača velike molekulske mase. Kao pospješivač apsorpcije korišten je kitozan, kationski polimer koji reverzibilno otvara čvrste međustanične veze. Kao obilježivač paracelularnog prijenosa korišten je dekstran FD-4, fluorescentni obilježivač velike molekulske mase. Ponašanje in vitro modela epitela rožnice uspoređeno je s ponašanjem in vitro modela crijevnog epitela. Mjerenjem transepitelnog električnog otpora potvrđen je učinak kitozana na otvaranje čvrstih veza in vitro epitelnog modela rožnice. Učinka kitozana na otvaranje čvrstih veza bio je ovisan o koncentraciji kitozana. Primjenom kitozana došlo je do povećanja prividnog koeficijenta permeabilnosti (Papp) dekstrana FD-4. Citotoksični učinak kitozana na stanice in vitro modela epitela rožnice isključen je praćenjem metaboličke aktivnosti.
Abstract (english)
This diploma thesis is a part of research project which main goal is the development and implementation of high throughput in vitro corneal epithelial model characterized by good predictability of eye-related bioavailability. The aim of this diploma thesis was to investigate the applicability of in vitro corneal epithelial model for studying the paracellular transport. For that purpose, it was necessary to investigate the effect of absorption enhancers which mechanism of action is based on the opening of tight junctions on the permeability of paracellular markers. Chitosan was chosen as the absorption enhancer due to its known effect of reversible opening of tight junctions. Dextran FD-4 was chosen as the fluorescent macromolecular marker of paracellular transport. The behaviour of in vitro corneal epithelial model was compared with the behaviour of in vitro model of intestinal epithelium. The effect of chitosan was monitored by measuring transepithelial electrical resistance. The effect of chitosan was concentration dependent. Chitosan induced the enhancement of the apparent permeability coefficient of dextran FD-4. Cytotoxic effect of chitosan was excluded by monitoring the cell metabolic activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:758402
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-02-22 11:38:42