Artichoke (Cynara scolymus), belonging to the family of Asteraceae, is an herbaceous perennial crop, widely cultivated in the Mediterranean area. The heads of the artichoke are edible and used worldwide, while the leaves are an herbal medicine recognized far a long time far their beneficial and therapeutic effects, including induction of choleresis, LDL oxidation and significant antioxidant, antifungal, antibacterial as well as strong hepatoprotective activity. Artichoke contains very little fat and high levels of minerals, vitamins, fibre, inuline and polyphenols. The most abundant phenolic compounds reported in artichoke leaf are flavonoides such as apigenin and luteolin (both glycosides and rutinosides), as well as phenolic acids, particularly derivatives of mono- and di-caffeoylquinic acid. Although all polyphenols are included in cholesterol lowering effect of artichoke leaf, the most responsible one is cynarin, derivative of dicaffeoylquinic acid. As dietary supplements have an important position in health care systems worldwide, their current assessment and quality control are a major bottleneck. Over the last years, major steps were taken to improve the quality of the dietary supplements and also to develop analytical methods ensuring their quality. High performance liquid chromatography has become the most popular analytical technique far analyzing dietary supplements. The main advantages of the technique are high resolution, selectivity, sensitivity and possibility to couple the technique to different detectors. Therefore, the aim of the present work was to develop a new HPLC! DADIMS/MS method far identification of cynarin present in artichoke leaf extract tablets.