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Scientific paper - Original scientific paper
Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon
Farmaceutski glasnik, 65 (2009), 7-8; 407-415. urn:nbn:hr:163:404463
Tomašek, Ljubica; Žuntar, Irena; Đirlić, Jasenka; Mršić, Gordan

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Tomašek, Lj., Žuntar, I., Đirlić, J. & Mršić, G. (2009). Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon. Farmaceutski glasnik, 65. (7-8), 407-415. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:404463

Tomašek, Ljubica, et al. "Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon." Farmaceutski glasnik, vol. 65, no. 7-8, 2009, pp. 407-415. https://urn.nsk.hr/urn:nbn:hr:163:404463

Tomašek, Ljubica, Irena Žuntar, Jasenka Đirlić and Gordan Mršić. "Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon." Farmaceutski glasnik 65, no. 7-8 (2009): 407-415. https://urn.nsk.hr/urn:nbn:hr:163:404463

Tomašek, Lj., et al. (2009) 'Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon', Farmaceutski glasnik, 65(7-8), pp. 407-415. Available at: https://urn.nsk.hr/urn:nbn:hr:163:404463 (Accessed 27 December 2024)

Tomašek Lj, Žuntar I, Đirlić J, Mršić G. Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon. Farmaceutski glasnik [Internet]. 2009 [cited 2024 December 27];65(7-8):407-415. Available at: https://urn.nsk.hr/urn:nbn:hr:163:404463

Lj. Tomašek, I. Žuntar, J. Đirlić and G. Mršić, "Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon", Farmaceutski glasnik, vol. 65, no. 7-8, pp. 407-415, 2009. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:404463. [Accessed: 27 December 2024]

Metadata
Title (croatian)Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon
AuthorLjubica Tomašek
AuthorIrena Žuntar
AuthorJasenka Đirlić
AuthorGordan Mršić
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract (english)
Thin-layer chromatography is a quick, simple and economic analytical method for separating different analytes. Hence the purpose of this work was to validate thin-layer chromatography as an analytical procedure allowing simultaneous determination of the benzodiazepines alprazolam, diazepam, oxazepam and the opioid methadone. Validation of testing quality was performed using two TLC systems: TA system involving methanol and ammonium mixed in a 100:1.5 ratio and Te system with dichloromethane and methanol mixed in a 9:1 ratio. In the process of validating the testing quality, selectivity needs to be tested and limit of detection must be set for each analyte. For selectivity testing, standard analyte solutions and certain auxiliary substances were prepared (matrix), so we could monitor the influence of the matrix on analyte separation. Limit of detection was established by preparing a set of dilutions of basic standard solutions; after chromatogram visualisation, the lowest concentration for detecting an analyte was set. Limits of detection are: alprazolam 0.80 μg/mL for TA system and 0.53 μg/mL for Te system; diazepam 3.14 μg/mL for TA and Te systems; oxazepam 3.53 μg/mL for TA system and 5.30 μg/mL for Te system, and methadone 6.09 μg/mL for TA system and 9 .14 μg/mL for Te system. Based on the validation results, we were able to confirm that TLC systems TA and Te are appropriate for identification of benzodiazepines like alprazolam, diazepam, oxazepam and of methadone and that auxiliary substances have no effect on the identification process.
Keywords (croatian)
Languagecroatian
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - domestic
Publication versionPublished version
Journal titleFarmaceutski glasnik
Numberingvol. 65, no. 7-8, pp. 407-415
p-ISSN0014-8202
URN:NBNurn:nbn:hr:163:404463
Publication2009
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-10-07 11:14:30