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master's thesis
Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:618959
Lenić, Ines
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical analysis

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Lenić, I. (2015). Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:618959

Lenić, Ines. "Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:618959

Lenić, Ines. "Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:618959

Lenić, I. (2015). 'Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:618959

Lenić I. Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:163:618959

I. Lenić, "Microchip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:618959

Metadata
TitleMicrochip electrophoresis-electrochemical detection platform for analysis of drugs and their metabolites
AuthorInes Lenić
MentorBiljana Nigović (mentor)
Committee memberBiljana Nigović
Committee memberAna Mornar Turk
Committee memberLidija Bach-Rojecky
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical analysis)
Zagreb
Defense date and country2015-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
U razdoblju ubrzanog rasta farmaceutske industrije razvoj postojećih analitičkih metoda je prespor i ne može zadovoljiti potrebe zahtjevnih metaboličkih analiza novih lijekova i novih kemijskih entiteta (kemikalija iz okoliša i kućanstva). Prenosiva platforma za mikročipnu kapilarnu elektroforezu (MCE) s amperometrijskom elektrokemijskom detekcijom ispitana je u ovom radu i nudi novi pristup u području analitičke kemije. Prateći koncept Micro Total Analysis System (μTAS) ili mikro sustava za kompletne analize kombinira brzinu, nisku cijenu i praktičnost s niskim potrebama reagenasa. Istraživanje je provedeno na kompaktnoj platformi za MCE s elektrokemijskom detekcijom nabavljenoj od tvrtke Micrux Technologies. Metoda je razvijena za odabrane parove lijekova i metabolita (fenacetin/paracetamol, kumarin/umbeliferon, diklofenak/4'-hidroksidiklofenak). Ispitani su različiti naponi, vremena analiza i postavke sustava. Također, različite metode implementirane su na mikročip s ciljem pripreme i pročiščavanja uzorka (mikroekstrakcija na tekućoj fazi i elektrokinetičko slaganje uzorka). Određeno je područje linearnosti analitičke metode te limit detekcije i limit kvantifikacije za svaki od supstrata.
Abstract (english)
In a period of rapidly growing pharmaceutical industry development of the current analytical techniques is by far too slow to fulfil requests of demanding metabolic analysis of new pharmaceuticals and new chemical entities (environmental and household chemicals). Portable Microchip Capillary Electrophoresis (MCE) platform with amperometric electrochemical detection (EC) was developed in this work, offering a new approach to analytical chemistry. By following Micro Total Analysis System (μTAS) concept, MCE-EC platform combines rapid analysis, low price and practicality with low requirements of reagents. Research was made by using compact platform for MCE-EC purchased from MicruX Techologies. It was developed for chosen drug/metabolite pairs (phenacetin/paracetamol, coumarin/umbelliferone and diclofenac/4'-hydroxydiclofenac). Different voltages, analysis times and system setups were tested. In addition to this, different on-chip sample preparation and purification methods (liquid phase microextraction and electrokinetic sample stacking) were implemented on this easy-handle platform. The linearity of an analytical procedure, as well as the limit of detection (LOD) and the limit of quantification (LOQ) were determined.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:163:618959
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-02-24 16:19:00