prikaz prve stranice dokumenta Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima
  Download
PDF 1.7 MB
master's thesis
Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:243481
Čuljak, Sandra
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Čuljak, S. (2015). Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:243481

Čuljak, Sandra. "Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:243481

Čuljak, Sandra. "Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:243481

Čuljak, S. (2015). 'Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:243481

Čuljak S. Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:243481

S. Čuljak, "Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:243481

Metadata
TitleHipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima
Title (english)Hypoglycemic and hepatoprotective effect off genus Globularia in HepG2 cells under hyperglycemic conditions
AuthorSandra Čuljak
MentorRoberta Petlevski (mentor)
Committee memberRoberta Petlevski
Committee memberŽeljka Vanić
Committee memberLovorka Vujić
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2015-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Tretiranje HepG2 stanica 20 mM glukozom uzrokuje hiperglikemijske uvjete koji dovode do oštećenja hepatocita što se očituje porastom jetrenih enzima (ALT i AST). Cilj ovog rada bio je ispitati hepatoprotektivni učinak Globularia spp na aktivnost jetrenih enzima (ALT i AST), ispitati učinak Globularia spp na poboljšanje funkcionalne sposobnosti jetre (sinteza albumina) te učinak na inhibiciju enzima a-glukozidaze, enzima koji katalizira pretvorbu oligosaharida i disaharida u monosaharide. Inhibicijom tog enzima postiže se hipoglikemijski učinak. Aktivnosti enzima izmjereni su u lizatu stanica. Istraživanje je pokazalo statistički značajno povećanje aktivnosti enzima (ALT i AST) u lizatu HepG2 stanica tretirani 20 mM glukozom u odnosu na kontrolnu skupinu (P<0,05). Tretiranje HepG2 stanica Globularia spp (0,5 i I mg/mL) i 20 mM glukozom statistički je značajno smanjilo aktivnost enzima ALT u odnosu na skupinu tretiranu samo 20 mM glukozom. Nije pokazano statistički značajno smanjenje aktivnosti AST nakon tretiranja različitim Globularia spp. Iako je uočen porast u sintezi albumina kod stanica tretiranih Globularia spp taj porast nije statistički značajan. Rezultati ispitivanja učinka Globularia spp na inhibiciju a-glukozidaze pokazali su pozitivan učinak tj postignuta je statistički značajna inhibicija a-glukozidaze.
Abstract (english)
Treatmet of HepG2 cells with 20 mM glucose causes hyperglycemic conditions , which leads to damage on hepatocytes as evidenced by increased activitiy of liver enzymes (ALT, AST). The aim oft this work is to investigate hepatoprotective effect of Globularia spp on the activity of liver enzymes , effect Globularia spp on the albumin synthesis, and effect Globularia spp on the activitiy of inhibition a-glucosidase. Activities of these enzymes were measured in cell lysates. Treatment of cells with 20 mM glucose significantly (P<0,05) increased activities of liver enzymes compared to the control group. Treatment with 20 mM glucose and Globularia spp significantly {P<0,05) decreased activities of liver enzymes comperd to the group treated only with 20 mM glucose, while treatment with Globularia spp did not decreased activities of liver enzymes. Treatment of cells with Globularia spp did not showed statistically significant (P<0,05) increased albumin synthesis. Treatment of cells with Globularia spp significantly {P<0,05) showed a-glucosidase inhibitory activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:243481
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-02-29 15:03:44