prikaz prve stranice dokumenta Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije
  Download
PDF 1.06 MB
master's thesis
Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. urn:nbn:hr:163:611747
Kovačević, Ivana
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of analytical chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Kovačević, I. (2020). Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:611747

Kovačević, Ivana. "Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:611747

Kovačević, Ivana. "Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:611747

Kovačević, I. (2020). 'Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:611747

Kovačević I. Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2020 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:163:611747

I. Kovačević, "Određivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:163:611747

Metadata
TitleOdređivanje bakra u ljudskom urinu primjenom atomske apsorpcijske spektrofotometrije
Title (english)Determination of copper in human urine with use of atomic absorption sprectrophotometry
AuthorIvana Kovačević
MentorSuzana Inić (mentor)
Committee memberSuzana Inić (predsjednik povjerenstva)
Committee memberJasna Jablan (član povjerenstva)
Committee memberMirela Matić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of analytical chemistry)
Zagreb
Defense date and country2020-10-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Bakar je jedan od esencijalnih elemenata u tragovima i iznimno je vaţan za mnoge biološke procese u ljudskom organizmu. Promjena njegove koncentracije u tjelesnim tekućinama i organima moţe izazvati brojne patofiziološke poremećaje. Stoga je određivanje bakra u biološkim uzorcima vaţno za otkrivanje bolesti uzrokovanih promjenom njegove koncentracije u organizmu. U ovom diplomskom radu određena je koncentraciju bakra u uzorcima urina primjenom atomske apsorpcijske spektrofometrije (AAnalyst 800, Perkin Elmer Instruments, Norwalk, CT, SAD) na valnoj duljini od 324,8 nm. Analitička metoda je validirana korištenjem standardnih otopina bakra u rasponu koncentracija od 0,25 do 3,0 mg/L. Metoda je bila linearna u ispitivanom području (R2 = 0,9999). Ponovljivost metode iskazana kao relativna standardna devijacija (RSD) bila je do 3,5 %, a srednja preciznost iskazana kao RSD bila je do 5%. Granica dokazivanja (LOD) iznosila je 0,0054 mg/L, a granica određivanja (LOQ) 0,0163 mg/L što ukazuje na osjetljivost metode. Koncentracija bakra u sedam uzoraka urina zdravih ispitanika bila je u rasponu 0,0168 – 0,0546 mg/L. Uvedena i validirana AAS metoda se pokazala prikladnom za osjetljivo, precizno i brzo određivanje koncentracije bakra u ljudskom urinu.
Abstract (english)
Copper is one of the essential trace elements and it is very important for many biological processes in the human body. Change in the concentration of copper in body fluids and organs can lead to numerous pathophysiological disorders. Therefore, the determination of copper in biological samples is important to detect diseases caused by changes its concentration in the body. In this thesis, concentration of copper in urine samples was determined by atomic absorption sprectrophotometry (AAnalyst 800, Perkin Elmer Instruments, Norwalk, CT, SAD) at wavelenght of 324,8 nm. The method was validated using a copper standard in the concentration range of 0,25 to 3.0 mg/L. The method was linear in test area (R²=0,9999). The repeatability of the method, expressed as relative standard deviation (RSD), was up to 3,5% and intermediate precision expressed as RSD was under 5%. The limit of detection (LOD) was 0,0054 mg/L, and limit of quantification (LOQ) was 0,0163 mg/L, that indicating the sensitivity of the method. The concentration of copper, measured in seven healthy human urine samples were in the range from 0.0168 to 0.0546 mg/L. The developed and validated AAS method is suitable for an accurate, precise and fast determination of copper in the human urine.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:611747
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-04-23 12:52:53