It is believed that there is a link between diabetes and oxidative stress, and that oxidative stress is a major cause in the development of diabetes through effects on insulin resistance, glucose tolerance and beta-cells. It is also believed that oxidative stress is involved in the development of diabetic complications. Consequently, there is possibility that a therapy which aims to reduce oxidative stress may be of use to those suffering from diabetes, as well as those who are at increased risk. Hyperglycemia affects the development of oxidative stress through several mechanisms such as auto-oxidation of glucose, non-enzymatic glycation of proteins, oxidative modification of metalloproteins, etc. People with diabetes in a long-term can develop a series of microvascular kidney, retina and nerve diseases. Flavonoids are a large group of polyphenolic compounds of the benzo-ă-pyrone structure and are found in plants. The flavanon naringenin is contained in citrus fruits, especially in grapefruit, and tomatoes, and the cocoa. It affects human health as antioxidant and radical scavenger, has anti-inflammatory properties, improves carbohydrate metabolism, and modulates the immune system. The aim of this study was to examine the effect of naringenin at low concentrations (1000, 100, 10, and 1 ěg/mL) on the viability of HepG2 liver cells in hyperglycemic conditions by lactate dehydrogenase (LDH) and MTT assay. To achieve the condition of hyperglycemia and oxidative stress in HepG2 cells, the cells are treated with a 30 mM glucose solution. Treatment of cells by low concentrations of naringenin led to a statistically significant (P <0.05) decrease in activity of lactate dehydrogenase (LDH) in all naringenin-treated groups compared to the untreated diabetic group. However in the case of the MTT assay treatment by low concentrations of naringenin did not result in statistically significant differences (P <0.05) between the cells treated with naringenin and untreated diabetic group. Results of this study indicate that naringenin has a positive effect on the viability of Hep G2 liver cells in hyperglycemic conditions at low concentrations of naringenin.