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master's thesis
Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. urn:nbn:hr:163:044719
Šarčević, Željka
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Šarčević, Ž. (2020). Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:044719

Šarčević, Željka. "Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:044719

Šarčević, Željka. "Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:044719

Šarčević, Ž. (2020). 'Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:044719

Šarčević Ž. Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2020 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:163:044719

Ž. Šarčević, "Usporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:163:044719

Metadata
TitleUsporedba koncentracija fenilalanina i slobodnog karnitina u uzorcima suhe kapi krvi, plazme i suhe kapi plazme na tandemskom spektrometru masa
Title (english)Comparison of phenylalanine and free carnitine concentrations in dried blood spots, plasma and dried plasma spots using tandem mass spectrometry
AuthorŽeljka Šarčević
MentorKsenija Fumić (mentor)
Committee memberKsenija Fumić (predsjednik povjerenstva)
Committee memberPetra Turčić (član povjerenstva)
Committee memberOlga Gornik Kljaić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2020-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
U svrhu provođenja novorođenačkog probira u uzorcima suhe kapi krvi određuje se profil aminokiselina i acilkarnitina. Prednost suhe kapi krvi i suhe kapi plazme u odnosu na uzorak plazme/seruma je manji volumen uzorkovanja, stabilnost analita na sobnoj temperaturi, lakši transport i pohrana. Međutim, faktori koji utječu na kvantitativni rezultat analize izrezanog isječka iz uzorka suhe kapi krvi su volumen krvi koji se aplicira na filtar papir, hematokrit, distribucija analita u suhoj kapi zbog utjecaja eritrocita i postupak ekstrakcije analita. Za pacijente s potvrđenim metaboličkim poremećajima koji se kontinuirano prate, velika je prednost što uzorke suhe kapi krvi koju mogu poslati poštom u laboratorij i smanjiti učestalost i invazivnost venepunkcije stalnih pretraga. Međutim, za većinu testova koji se koriste u praćenju metaboličkih pacijenata primarni uzorak izbora je plazma. Cilj ovog rada bio je usporediti koncentracije fenilalanina i slobodnog karnitina u uzorcima suhe kapi plazme (Noviplex®) i suhe kapi krvi te uzorcima suhe kapi plazme i uzorcima plazme, a u svrhu poboljšanja dijagnostike i praćenja pacijenata s nasljednim metaboličkim poremećajima. Koncentracije fenilalanina i slobodnog karnitina mjerene su komercijalnim kompletom reagensa tvrtke Recipe na tandemskom spektrometru masa tvrtke Shimadzu. Statističkim testovima pokazano je da ne postoji statistički značajna razlika u koncentraciji fenilalanina, kao ni u koncentraciji slobodnog karnitina, u uzorcima suhe kapi plazme i uzorcima suhe kapi krvi, dok je pokazano da postoji statistički značajna razlika u koncentraciji fenilalanina kao i u koncentraciji slobodnog karnitina u uzorcima suhe kapi plazme i uzorcima plazme. Uz uključivanje većeg broja ispitanika i većeg raspona koncentracija, trebalo bi ispitati i različite načine ekstrakcije analita iz uzorka suhe kapi plazme i plazme. Naime, do tog zaključka navode nas neslaganja u koncentracijama fenilalanina i slobodnog karnitina između uzoraka suhe kapi plazme i uzoraka plazma, dok uzorci suhe kapi krvi i suhe kapi plazme pokazuju zadovoljavajuće rezultate.
Abstract (english)
Amino acid and acylcarnitine profiles are determined from dried blood samples in purpose of newborn screening. The advantages of dried blood spots and dried plasma spots in comparison with plasma/serum samples are lesser volume used in sampling, room temperature analyte stability, easier transport and storage. However, factors which influence the quantitative result analysis of the cut-out disk from dried blood sample are blood volume applied on the filter paper, hematocrit, analyte distribution affected by erythrocytes and analyte extraction process. Metabolic disorders patients are required continuous testing, and for them it is convenient to send dried blood samples by mail and decrease the number of hospital visits and invasiveness of venipuncture. However, most of the tests used for metabolic patients require a plasma sample. The aim of this study was to compare concentrations of phenylalanine and free carnitine in dried plasma spots (Noviplex®) and dried blood spots as well as dried plasma spots and plasma samples, in purpose of improvements in diagnostics and monitoring metabolic disorders patients. Phenylalanine and free carnitine concentrations were measured using commercial reagent kits made by Recipe on tandem mass spectrometer by Shimadzu. Statistic tests have shown no significant statistical difference in phenylalanine concentrations, as well as free carnitine concentrations in dried plasma spots and dried blood spots. However, there has been statistically significant difference in phenylalanine concentrations as well as free carnitine concentrations in dried plasma spots and regular plasma samples. Along with including a higher number of participants and a wider concentration range, different methods for extracting analytes from dried plasma spots and regular plasma samples should be tested. Namely, disagreement in phenylalanine and free carnitine concentrations between dried plasma spots and regular plasma samples lead to this conclusion, while dried blood spots and dried plasma spots show satisfying results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:044719
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije i laboratorijske medicine (magistar/magistra medicinske biokemije i laboratorijske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-04-27 15:39:19