HIL indices are the result of automatic detection of haemolysis, lipemia and icterus on automatic analysers. They are determined on most biochemistry and coagulation analysers and have become the golden standard in sample quality evaluation. Only recently HIL indices have been introduced on coagulation analysers, they are rarely used in routine work and there have not been many verification studies regardless of the fact that haemolysis, lipemia and icterus influence coagulation assays. Therefore, the main goal of this study was to verify the reliability of the HIL indices assessment on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 (Siemens Healthcare, Marburg, Germany).The reliability of the HIL indices assessment was examined by comparison of qualitative HIL indices assessment results on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 with the results of the quantitative HIL indices assessment on biochemistry analyser Cobas c501 (Roche Diagnostics, Mannheim, Germany) and with the exact concentrations of free haemoglobin for H index, of triglycerides for L index and of total bilirubin for I index in analysed samples. Main findings of this study are that the HIL indices assessment results on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 are in correlation with measured interference concentrations and with quantitative HIL indices on Cobas c501; further, that both analysers detect very low concentrations of interferences and that the HIL indices on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 are reliable for usage in everyday routine practice.