The aim of this study was to examine the release profile of azithromycin from liposomes embedded in chitosan hydrogel. For that purpose, two type of cationic liposomes were prepared by film hydration method: DPPC/DODAB liposomes and SPC liposomes coated with 0,3% (w/w) chitosan solution. Negatively charged SPC liposomes served for comparison. All liposomal preparations were physically characterized before and after homogenization by measuring mean diameter, size distribution and zeta potential. Homogenization of different liposome dispersions was preformed using an ultrasonic probe sonicator or extruder. After separation of unencapsulated drug, the liposomes were mixed into a gel prepared from high-molecular weight chitosan. The concentration of liposomes in the gel was 30% (w/w). After homogenization, the mean diameter ranged between 130 nm (SPC liposomes) and 250 nm (DPPC/DODAB). Liposomes coated with chitosan were slightly higher due to the specificities of the manufacturing process. Zeta potentials of the cationic liposomes were higher than +30 mV, while the zeta potential of the SPC liposomes was less than -30 mV, indicating the physical stability of all the prepared liposome dispersions. In vitro release results indicate a trend of rapid drug release in the first 4 hours and slower in the next 20 hours. There were no statistically significant differences in the drug release from all the tested cationic and anionic liposomes. When compared to control (gel containing solution of azithromycin instead of the liposomes), slower release of azithromycin from all the tested liposome formulations was proven.