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doctoral thesis
Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. urn:nbn:hr:163:338856
Fumić, Barbara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmacognosy
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Fumić, B. (2020). Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:338856

Fumić, Barbara. "Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family." Doctoral thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry; University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:338856

Fumić, Barbara. "Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family." Doctoral thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry; University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:338856

Fumić, B. (2020). 'Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family', Doctoral thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry; University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:163:338856

Fumić B. Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2020 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:163:338856

B. Fumić, "Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family", Doctoral thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb; University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:163:338856

Metadata
TitleIsolation, solubilization and biological activity of isoflavonoids from Fabaceae family
Title (croatian)Izolacija, solubilizacija i biološka aktivnost izoflavona iz porodice Fabaceae
AuthorBarbara Fumić
MentorMarijana Zovko Končić (mentor)
MentorMario Jug (komentor)
Committee memberSanda Vladimir-Knežević (predsjednik povjerenstva)
Committee memberŽeljka Vanić (član povjerenstva)
Committee memberMila Lovrić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmacognosy)
Zagreb
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical technology)
Zagreb
Defense date and country2020-06-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Mucopolysaccharidoses are hereditary metabolic diseases characterized by the accumulation of various glycosaminoglycans in the tissues of the diseased. Isoflavones are secondary plant metabolites that can slow down the synthesis of glycosaminoglycans, but their therapeutic potential is significantly limited by their poor oral bioavailability. In this thesis, the ethanol and cyclodextrin ultrasound-assisted extraction of aerial parts of two plant species from the family Fabaceae, Medicago sativa and Lotus corniculatus, was compared. The study was conducted using response surface methodology based on a Box-Benkhen design with three independent variables. The influence of isoflavones, the prepared extracts and inclusion complexes of selected isoflavones with different cyclodextrins on oxidative stress and glycosaminoglycan synthesis in fibroblast cell lines of patients with mucopolysaccharidosis was evaluated. Although ethanol was a more suitable solvent for the extraction of total polyphenols from M. sativa, the use of hydroxypropyl--cyclodextrin allowed for a more selective extraction of total flavonoids whose amount was as much as three times higher than in ethanol extracts. The amount of total flavonoids extracted using cyclodextrin from L. corniculatus was two times greater than the amount of total flavonoids of the species M. sativa. The cyclodextrin complexes of these extracts showed, depending on the dose, a significant decrease in glycosaminoglycan levels in fibroblast cells of patients with mucopolysaccharidoses, with no significant cytotoxic effect.
Abstract (croatian)
Mukopolisaharidoze su nasljedne metaboličke bolesti koje karakterizira nakupljanje različitih glikozaminoglikana u tkivima oboljelih. Izoflavoni su sekundarni biljni metaboliti koji mogu usporiti sintezu glikozaminoglikana, ali je njihov terapijski potencijal značajno ograničen slabom oralnom bioraspoloživošću. U radu su uspoređene etanolna i ciklodekstrinska ultrazvučna ekstrakcija zeleni dvije biljne vrste iz porodice Fabaceae, Medicago sativa i Lotus corniculatus. Istraživanje je provedeno korištenjem metodologije površine odgovora temeljene na Box-Benkhen dizajnu s tri nezavisne varijable. Uspoređen je utjecaj izoflavona, priređenih ekstrakata te inkluzijskih kompleksa odabranih izoflavona s različitim ciklodekstrinima na oksidativni stres i sintezu glikozaminoglikana u staničnim linijama fibroblasta bolesnika oboljelih od mukopolisaharidoza. Iako je etanol bio prikladnije otapalo za ekstrakciju ukupnih polifenola iz M. sativa, uporaba hidroksipropil--ciklodekstrina omogućila je selektivniju ekstrakciju ukupnih flavonoida čija je količina bila čak tri puta veća nego u etanolnim ekstraktima. Količina ukupnih flavonoida ekstrahirana korištenjem ciklodekstrina iz biljnog materijala vrste L. corniculatus bila je još dva puta veća od količine ukupnih flavonoida iz vrste M. sativa. Ciklodekstrinski kompleksi navedenih ekstrakata pokazali su, ovisno o dozi, značajno sniženje razine glikozaminoglikana u stanicama fibroblasta bolesnika oboljelih od mukopolisaharidoza, bez značajnog citotoksičnog učinka.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:163:338856
Promotion2021
Study programmeTitle: Pharmacy and biochemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor znanosti (doktor znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-05-18 14:58:37