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master's thesis
Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:666534
Šujansky, Lara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical analysis

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Šujansky, L. (2019). Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:666534

Šujansky, Lara. "Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:666534

Šujansky, Lara. "Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:666534

Šujansky, L. (2019). 'Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:666534

Šujansky L. Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:666534

L. Šujansky, "Određivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:666534

Metadata
TitleOdređivanje antioksidativne aktivnosti analgetika HPLC-DPPH metodom
Title (english)Determination of antioxidant activity of analgesics by HPLC-DPPH method
AuthorLara Šujansky
MentorDaniela Amidžić Klarić (mentor)
Committee memberDaniela Amidžić Klarić (predsjednik povjerenstva)
Committee memberBiljana Nigović (član povjerenstva)
Committee memberCvijeta Jakobušić Brala (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical analysis)
Zagreb
Defense date and country2019-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Bol je složen, neugodan senzorni i emocionalni doživljaj koji se javlja kao direktni odgovor na stvarno ili potencijalno oštećenje tkiva. Analgetici se koriste u svrhu ublažavanja i uklanjanja boli kod niza stanja i bolesti različite etiologije. Obzirom na njihovu široku i čestu primjenu, korisno je ispitati imaju li predstavnici ove skupine lijekova dodatna mehanizme djelovanja poput antioksidativnog učinka. Cilj ovog rada bio je odrediti antioksidativnu aktivnost devet analgetika u in vitro uvjetima mjerenjem njihove sposobnosti hvatanja DPPH slobodnog radikala. U ovom radu je za procjenu antioksidativne aktivnosti 1 mM otopine analgetika korištena HPLC-DPPH metoda, koja se temelji na mjerenju sposobnosti hvatanja slobodnog radikala u prisustvu ispitivanog lijeka. Antioksidativni aktivnost izražen je kao ekvivalent standarda TROLOX-a. Dobiveni rezultati ukazuju na izraženu antioksidativnu aktivnost paracetamola. Korištena HPLC-DPPH metoda pokazala se jednostavnom, brzom i učinkovitom za ispitivanje antioksidativne aktivnosti slabih analgetika s ciljem ispitivanja njihovog dodatnog mehanizma djelovanja.
Abstract (english)
Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage. Analgesics are used for relieving or removing pain in number of conditions and illnesses with various etiology. Since they are widely and frequently used, it is useful to examine if they potentially have additional effects, especially antioxidant. The aim of this thesis was to examine antioxidant properties of nine nonsteroidal anti-inflammatory drugs (NSAIDs) by measuring their ability to scavenge DPPH radical in vitro. Method used for estimating antioxidant activity of 1 mM analgesic solutions in this research was HPLC-DPPH method, which is based on measuring the ability to scavenge free radicals in the presence of sample. Antioxidant capacity is expressed as the TROLOX equivalent. Experimental data indicate that paracetamol has antioxidant activity. The HPLC-DPPH method has been proven as simple, fast and efficient for antioxidant activity assays of NSAIDs with the purpose of examining their potential additional mechanisms of action.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:666534
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-07 13:55:04