prikaz prve stranice dokumenta Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor
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master's thesis
Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:468029
Kitić, Lana
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Kitić, L. (2019). Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:468029

Kitić, Lana. "Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:468029

Kitić, Lana. "Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:468029

Kitić, L. (2019). 'Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:468029

Kitić L. Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:163:468029

L. Kitić, "Markers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:468029

Metadata
TitleMarkers of immune cells, apoptosis and fibrosis in a porcine model of metabolic syndrome and a novel involvement of bile acid-activated receptor
Title (croatian)Markeri imunoloških stanica, apoptoze i fibroze u svinji kao modelu metaboličkog sindroma i doprinos receptora osjetljivog na prisustvo žučnih kiselina patogenezi srčanog zatajenja
AuthorLana Kitić
MentorJerka Dumić (mentor)
Committee memberJerka Dumić (predsjednik povjerenstva)
Committee memberSanja Dabelić (član povjerenstva)
Committee memberMaja Šegvić Klarić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2019-04-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Srčano zatajenje (SZ) definirano je kao negativna promjena u funkciji srca kao crpke, a s vremenom vodi k adaptivnim molekularnim i intersticijalnim promjenama čiji je razmjer još slabo istražen. Trenutna studija koristi tehniku imunofluorescencije kako bi ispitala tkivo lijevog atrija (LA) i lijevog ventrikula (LV) svinja koje su bile podređene hiperkaloričnoj i masnoj prehrani te razvile metabolički sindrom (MS) (n=5), kao i tkivo životinja kontrolne grupe (n=5). Cilj studije bio je korištenjem određenih staničnih markera utvrditi sudjelovanje upale (CD163, CD3, CD16), stanične smrti (TUNEL) i fibroze (vimentin) te receptora koji je osjetljiv na prisustvo žučnih kiselina (TGR5) u progresivnom smanjenju funkcije srčanog mišića. Rezultati su pokazali značajno povećanje ekspresije markera CD163 i CD16, ali ne i CD3 u oba tkiva životinja s MS. Zabilježeno je i povećanje stope apoptoze u životinja s MS. Porast ekspresije vimentina zabilježen je u lijevom ventrikulu bolesnih životinja, ali ne i u lijevom atriju. Konačno, značajan porast ekspresije TGR5 receptora zabilježen je u oba analizirana tkiva životinja s MS . Ova studija predstavlja važan test doprinosa žučnih kiselina i mehanizama kao što su upala, apoptoza i fibroza u patogenezi SZ koje prati MS. Dodatne studije potrebne su kako bi se točnije opisale promjene na staničnoj razini koje možebitno doprinose patogenezi SZ i time razvile nove terapijske opcije za ovu skupinu pacijenata.
Abstract (english)
Heart failure (HF) is defined as a syndrome caused by an inadequate performance of the heart as a pumping system. Over the course of time this leads to a series of adaptive molecular and interstitial changes, scope of which still remains to be elucidated. The current study utilized immunofluorescence staining technique to examine left atrial (LA) and left ventricular (LV) tissue in high-fat fed pigs which developed metabolic syndrome (MetS) (n=5), as well as in their healthy counterparts (n=5). The goal was to assess the involvement of inflammation (CD163, CD3, CD16), apoptosis (TUNEL), fibrosis (vimentin) and a bile acidactivated receptor (TGR5) in a progressive decrease in cardiac function observed in MetS patients. The results showed a significant increase in the expression of CD163 and CD16 but not CD3 in MetS animals. Further, there was an increase in apoptotic events in MetS animals compared to the control group. Vimentin expression was increased in LV tissue , but not in LA. Finally, a significant increase in TGR5 expression was evident in MetS animals compared to the control group. This study provides an important test of the involvement of bile acids, inflammation, apoptosis and fibrosis in the pathogenesis of HF. Additional studies are needed to elucidate the exact cellular shifts which occur during the HF so new therapeutic options could be developed for this group of patients.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:468029
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-08 09:25:08