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master's thesis
Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:999290
Pižir, Ivan
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of analytical chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Pižir, I. (2021). Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:999290

Pižir, Ivan. "Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:999290

Pižir, Ivan. "Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:999290

Pižir, I. (2021). 'Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:999290

Pižir I. Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:163:999290

I. Pižir, "Razvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:999290

Metadata
TitleRazvoj i validacija metode za određivanje metadona i metoklopramida u tabletama primjenom ramanove spektroskopije
Title (english)Development and validation of a method for determining of methadone and metoclopramide in tablets using raman spectroscopy
AuthorIvan Pižir
MentorJasna Jablan (mentor)
Committee memberJasna Jablan (predsjednik povjerenstva)
Committee memberMario Jug (član povjerenstva)
Committee memberIvana Bačić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of analytical chemistry)
Zagreb
Defense date and country2021-04-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Metadon je sintetski opioid koji se danas primarno koristi kao supstitucijska terapija u liječenju opioidne ovisnosti te se sve više istražuje, ali i koristi u liječenju kronične boli. Metoklopramid je primarno indiciran u odraslih za prevenciju i simptomatsko liječenje mučnine i povraćanja induciranih radioterapijom i kemoterapijom. Problem je što metoklopramid čini gotovo polovinu potrošnje lijekova za funkcionalne GIT poremećaje, a indiciran je samo kao dodatna terapija. Svrha ovog diplomskog rada bila je razviti i validirati brzu i jednostavnu metodu za određivanje metadona i metoklopramida u tabletama primjenom disperzivne Ramanove spektroskopije, a zatim ispitati razvijenu metodu u određivanju udjela metadona i metoklopramida u komercijalno dostupnim tabletama. Preliminarnim ispitivanjima odabran je laser 785 nm i laktoza kao najbolje punilo. Kod validacije analitičke metode ispitivani su parametri: linearnost, homogenost, preciznost (ponovljivost i srednja preciznost), točnost te granica određivanja i dokazivanja. Rezultati provedenih validacijskih mjerenja za sve parametre većinom su se nalazili unutar postavljenih granica, a svako odstupanje se može objasniti pogreškom analitičara prilikom pripreme uzorka. Primjenom validirane metode na realne uzorke, dobivene su vrijednosti metadona i metoklopramida u uzorcima tableta te su uspoređene s deklariranim. U daljnjim istraživanjima primjenjivati će se kemometrijski pristup analizi podataka zbog bolje kvantifikacije djelatnih tvari u uzorcima.
Abstract (english)
Methadone is a synthetic opioid used primarily as replacement therapy in the treatment of opioid dependence and is increasingly being researched, but it is also used in the treatment of chronic pain. Metoclopramide is primarily indicated in adults for the prevention and symptomatic treatment of nausea and vomiting induced by radiation and chemotherapy. Problematically, metoclopramide accounts for almost half of drug use in functional GIT disorders, although it is only indicated as adjunctive therapy. The aim of this work was to develop and validate a fast and simple method for the determination of methadone and metoclopramide in tablets using dispersive Raman spectroscopy, and then to investigate the developed method in the determination of the proportion of methadone and metoclopramide in tablets. In preliminary experiments, a 785 nm laser and lactose were selected as the best filler. In the validation of the analytical method, the following parameters were examined: linearity, homogeneity, precision (repeatability and intermediate precision), accuracy and limits of detection and quantification. The results of the validation measurements performed for all parameters, are mostly within the specified limits, with deviations due to errors made by analyst during sample preparation. Using a validated method on real samples, the values of the content of methadone and metoclopramide in tablet samples were determined and compared with the declared content. In further studies, a chemometric approach to data analysis will be applied to achieve better quantification of the active ingredients in the samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:999290
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-11-16 15:34:06