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master's thesis
Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:392756
Vujnović, Marina
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmacognosy

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Vujnović, M. (2019). Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:392756

Vujnović, Marina. "Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:392756

Vujnović, Marina. "Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:392756

Vujnović, M. (2019). 'Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:392756

Vujnović M. Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:392756

M. Vujnović, "Ispitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:392756

Metadata
TitleIspitivanje inhibicije kolagenaze i antioksidativne aktivnosti odabranih biljnih vrsta
Title (english)Examination of collagenase inhibition and antioxidant activity of selected plant species
AuthorMarina Vujnović
MentorMarijana Zovko Končić (mentor)
Committee memberMarijana Zovko Končić (predsjednik povjerenstva)
Committee memberMaja Bival Štefan (član povjerenstva)
Committee memberLovorka Vujić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmacognosy)
Zagreb
Defense date and country2019-11-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Biljne vrste intenzivno se istražuju zbog svojih mnogobrojnih i poznatih korisnih učinaka na kožu topikalnom primjenom. U ovom diplomskom radu ispitani su listovi biljne vrste Olea europaea te zeleni biljnih vrsta Medicago sativa, Medicago lupulina, Achillea millefolium, Plantago lanceolata, Plantago major i Centaurea jacea. Uspoređivani su etanolni ekstrakti različitih koncentracija (10%, 50% i 90%). Cilj je bio utvrditi njihovu sposobnost inhibicije kolagenaze i odrediti njihovu antioksidativnu aktivnost. Antioksidativna aktivnost ekstrakata ispitana je β-karoten linoleatnom analizom i ORAC metodom, dok je inhibicija kolagenaze ekstraktima ispitana pomoću ninhidrinskog reagensa. Ispitivanja su pokazala da svih sedam biljnih vrsta ima dobar antioksidativni potencijal. Kao najučinkovitije možemo izdvojiti biljne vrste M. sativa i M. lupulina i P. major. Utvrđeno je da ekstrakti pripremljeni s 10% i 90%-tnim etanolom imaju bolju učinkovitost od onih pripremljenih s 50%-tnim etanolom. Nadalje ispitivanja su pokazala da najveći potencijal za inhibiranje kolagenaze ima biljna vrsta P. major. Najbolja inhibicija kolagenaze je utvrđena kod ekstrakta pripremljenog s 10%-tnim etanolom.
Abstract (english)
Plants have been extensively researched for its numerous and well-known beneficial effects on topical skin application. This thesis examined the leaves of the plant species Olea europaea and aerial parts of Medicago sativa, Medicago lupulina, Achillea millefolium, Plantago lanceolata, Plantago major and Centaurea jacea. Ethanol extracts of different concentrations (10%, 50% and 90%) were compared. The aim was to determine their collagenase inhibition potential and to determine their antioxidant activity. The antioxidant activity of the extracts was tested by using β-carotene linoleic acid assay and ORAC method, while the inhibition of collagenase was tested by using ninhydrin reagent. The results have shown that all of these seven plant species have a good antioxidant potential. The most efficient were M. sativa L.and M. lupulina L., Fabaceae, as well as P. major L., Plantaginaceae. We have also found that the extracts prepared using 10% and 90% ethanol were more efficient in the applied assays than those prepared using 50% ethanol. Furthermore, the results have shown that the best collagenase inhibition potential has a plant species P. major L., Plantaginaceae. Also the most efficient inhibition of collagenase was found in extracts prepared using 10% ethanol.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:392756
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-01-21 15:53:04