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master's thesis
Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:145645
Veltruski, Sanja
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Veltruski, S. (2021). Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:145645

Veltruski, Sanja. "Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:145645

Veltruski, Sanja. "Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:145645

Veltruski, S. (2021). 'Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:145645

Veltruski S. Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:145645

S. Veltruski, "Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:145645

Metadata
TitleIspitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin
Title (english)Stability testing of fecal calprotectin extract using QUANTA Flash Calprotectin commercial extraction system
AuthorSanja Veltruski
MentorDonatella Verbanac (mentor)
Committee memberDonatella Verbanac (predsjednik povjerenstva)
Committee memberRoberta Petlevski (član povjerenstva)
Committee memberAndrea Tešija Kuna (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2021-11-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Fekalni kalprotektin je važan biomarker za razlikovanje upalnih crijevnih bolesti (IBD, engl. inflamatory bowel disease) od sindroma iritabilnog crijeva (IBS, engl. irritable bowel syndrome), kao i za praćenje tijeka bolesti i recidiva. Metoda je neinvazivna, a pacijent se upućuje na kolonoskopiju u slučaju povišene koncentracije fekalnog kalprotektina odnosno, ukoliko se sumnja na IBD. U ovom radu ispitana je stabilnost 20 uzoraka tijekom 120 sati. Koncentracija fekalnog kalprotektina se mjerila kemiluminiscentnom imunoanalizom (CLIA) upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash® Calprotectin (INOVA Diagnostics, San Diego, CA, USA) na analizatoru BIO-FLASH (INOVA Diagnostics, San Diego, CA, USA). Zaključno, 85% ekstrakata koji su priređeni i obrađeni po uputama proizvođača zadovoljili su traženim uvjetima stabilnosti.
Abstract (english)
Fecal calprotectin is an essential biomarker for distinguishing inflammatory bowel disease (IBD) from irritable bowel syndrome (IBS) and monitoring the course of disease and relapse. The method is non-invasive, and the patient is referred for colonoscopy in case of elevated fecal calprotectin concentration, particularly if IBD is suspected. In this experiment, the stability of 20 samples over 120 hours was examined. Fecal calprotectin concentration was determined by chemiluminescent immunoassay (CLIA) method using a commercial QUANTA Flash® Calprotectin extraction system (INOVA Diagnostics, San Diego, CA, USA) on a BIO-FLASH analyzer (INOVA Diagnostics, San Diego, CA, USA). Finally, 85% of the extracts prepared and processed according to the manufacturer's instructions meet the required stability requirements.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:145645
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije i laboratorijske medicine (magistar/magistra medicinske biokemije i laboratorijske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-02-09 10:39:01