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master's thesis
Primjena antisens peptida kao novi pristup liječenja ugriza poskoka
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:951925
Marić, Marin
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of general and inorganic chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Marić, M. (2021). Primjena antisens peptida kao novi pristup liječenja ugriza poskoka (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:951925

Marić, Marin. "Primjena antisens peptida kao novi pristup liječenja ugriza poskoka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:951925

Marić, Marin. "Primjena antisens peptida kao novi pristup liječenja ugriza poskoka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:951925

Marić, M. (2021). 'Primjena antisens peptida kao novi pristup liječenja ugriza poskoka', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:951925

Marić M. Primjena antisens peptida kao novi pristup liječenja ugriza poskoka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:163:951925

M. Marić, "Primjena antisens peptida kao novi pristup liječenja ugriza poskoka", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:951925

Metadata
TitlePrimjena antisens peptida kao novi pristup liječenja ugriza poskoka
Title (english)Antisens peptides application as a new approach to horned viper envenomation treatment
AuthorMarin Marić
MentorMario Gabričević (mentor)
Committee memberMario Gabričević (predsjednik povjerenstva)
Committee memberTin Weitner (član povjerenstva)
Committee memberViktor Pilepić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of general and inorganic chemistry)
Zagreb
Defense date and country2021-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Danas se kao terapija ugriza poskoka koriste imunoglobulini zmijskog protuotrova čija je proizvodnja složena, skupa i dugotrajna. Kao novi pristup liječenju predlaže se upotreba oligopeptida koji će se nekovalentno vezati na dijelove amoditoksina A odgovorne za neurotoksičnost inhibirajući njegovo djelovanje. Dizajn takvih oligopeptida temelji se na teoriji molekularnog prepoznavanja koja sugerira da svaka aminokiselina ulazi u interakciju s točno određenom aminokiselinom. Cilj ovog istraživanja je odrediti afinitet vezanja sens peptida koji predstavljaju neurotoksične dijelove amoditoksina A i njihovih potencijalno komplementarnih antisens peptida. Primarna struktura sens1 peptida je GMMILG, a njegovih antisens peptida SHHYEP i SHHYSP. Primarna struktura sens2 peptida je YIYRNYPDF, a njegovih antisens peptida INISIVGVK i VNISVIGIK. Konstante disocijacije određene su spektrofluorimetrijskim određivanjem intenziteta fluorescencije tijekom titracije. Kao titranti su korišteni sens1, sens2antisens1 i sens2antisens2 peptidi jer ne fluoresciraju zbog odsustva aromatskih aminokiselina. Kao titrandi su korišteni sens1antisens1, sens1antisens2 i sens2 peptidi koji fluoresciraju. Analizom eksperimentalno dobivenih podataka računalno su izračunate konstante disocijacija. Njihova vrijednost je usporediva s vrijednostima konstantama disocijacije većine antitijela, što upućuje na jaku interakciju, tj. afinitet vezanja. Kd za interakciju sens1 i njegovih antisens peptida su 5,071·10-6 M i 1,434·10-6 M, a za interakciju sens2 peptida i njegovih antisens peptida 1,143·10-6 M i 9,569·10-6 M. Navedeni rezultati potvrđuju vezanje sens i antisens peptida i potencijalni terapijski učinak antisens peptida koji se mogu koristiti u daljnjim istraživanjima. Pozitivan rezultat ovog istraživanja sugerira što veću upotrebu teorije molekularnog prepoznavanja za dizajniranje antisens peptida koji bi se mogli koristiti u raznim područjima biomedicine.
Abstract (english)
Nowadays snake antivenom imunoglobulins are being used for snake bite treatment whose production is complex, expensive and long. Use of oligopeptides is suggested as a new therapeutic approach which form noncovalent bonds with ammodytoxin A neurotoxic sites inhibiting it's action. Oligopeptide design is based on molecular recognition theory which indicates that every aminoacid can interact with the only specific complementary aminoacid. The aim of this research is the determination of binding affinity of sens peptides which represent ammodytoxin A neurotoxic sites and their potentially complementary antisens peptides. Primary structure of sens1 peptide is GMMILG and his antisens peptides are SHHYEP and SHHYSP. Primary structure of sens2 peptide is YIYRNYPDF and his antisens peptides are INISIVGVK and VNISVIGIK. Dissociation constants are determined by spectrofluorimetric measuring of fluorescence intensity during titration. Sens1, sens2antisens1 and sens2antisens2 peptides were used as titrants considering the fact they contain aromatic aminoacids which are responsible for fluorescence. On the other hand, sens1antisens1, sens1antisens2 and sens2 peptides were used as titrands since they lack aromatic aminoacids. Computer program analysed experimental data and calculated dissociaton constants. Their values are comparable to those of antibodies which points to strong interaction, i. e. binding affinity. Kd values for sens1 peptide and it's antisens peptides interactions are 5,071·10-6 M and 1,434·10-6 M and for sens2 and it's antisens peptides interactions are 1,143·10-6 M and 9,569·10-6 M. These results confirm sens and antisens peptides binding as well as potential antisens therapeutic effect which can be further used in future studies. Also, positive results of this research suggest greater use of molecular recognition theory for antisens peptide design which can be applied in various biomedicine fields.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:951925
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-02-09 11:37:02