Vaccination is unquestionably one of the greatest achievements of the last century, representing the most effective public health method in eradicating and preventing the spread of infectious diseases. Although the benefits of vaccination are well known, there is still a trend of insufficient vaccination coverage in influenza vaccination. It is estimated that 3 to 5 million people worldwide get the most severe form of the flu, 290.000 to 650.000 people do not survive the disease, while in Europe about 70.000. Vaccination has been allocated in some countries around the world to community pharmacists who have been recognized as important and most available health care professionals which had a positive effect on the overall coverage and role of pharmacists. This research aimed to determine the attitudes of pharmacists and patients in Croatia about the potential implementation of vaccination in community pharmacies and to determine the value of the service. This cross-sectional study was conducted from May to July 2021. Questionnaires were designed for the study and as such was used for the first time. On the Likert scale (1 - 5), respondents were able to express their opinion on offered statements. 362 pharmacists participated in the study, which is about 13% of the total number of pharmacists employed in community pharmacies. The sample consisted of 86.7% women and 13.3% men, and the median of pharmacists' age was 36.0 [16.0] years. About half of the respondents (55%) believe that vaccination should be provided in pharmacies. A significant correlation was observed between the attitudes that the patient should be provided with vaccination in pharmacies and readiness to perform services (𝜌 = 0.724, p < 0.01). Almost all pharmacists agree that they would not vaccinate without education, and after education more than 60% of pharmacists think differently. Over 90% of pharmacists agree that there should be an appropriate license for this service, which should be periodically renewed through professional training. 367 patients participated in the study. The sample consisted of 62,7% women and 37,3%. The median of patients' age was 41.0 [26.0]. Pharmacists and patients estimated median value of vaccination services of HRK 50, regardless of who financed it. In patients, a larger interquartile range was observed and shifted to a larger amount (35 - 100) than in pharmacists (20 - 70). In conclusion, almost all pharmacists involved in this study have a positive attitude towards vaccination in general and almost two thirds of pharmacists are willing to vaccinate patients in the pharmacy with prior education. On the other hand, we should not ignore the fact that almost half of pharmacists do not have a positive attitude or are undecided about the introduction of vaccination as a service in the pharmacy and that about a third believe that vaccination does not fall within pharmacists’ competencies. These results suggest the need for discussion within the profession about the role of the pharmacist, who is the most accessible healthcare professional, in vaccination.