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master's thesis
In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:102402
Šimović, Iva
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Šimović, I. (2022). In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:102402

Šimović, Iva. "In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:102402

Šimović, Iva. "In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:102402

Šimović, I. (2022). 'In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:102402

Šimović I. In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:163:102402

I. Šimović, "In vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:102402

Metadata
TitleIn vitro karakterizacija nazalnog praškastog sustava s natrijevim deksametazon fosfatom na Calu-3 staničnom modelu respiratornog epitela
Title (english)In vitro characterisation of nasal powder delivery system of sodium dexamethasone phosphate using Calu-3 respiratory epithelial cell model
AuthorIva Šimović
MentorAnita Hafner (mentor)
Committee memberAnita Hafner (predsjednik povjerenstva)
Committee memberMaja Ortner Hadžiabdić (član povjerenstva)
Committee memberBisera Jurišić Dukovski (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical technology)
Zagreb
Defense date and country2022-03-31, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Nazalna primjena lijekova istraţuje se u svrhu izravne dostave u središnji ţivčani sustav. Razvijanjem novih praškastih terapijskih sustava poboljšava se efikasnost takve primjene. U ranoj fazi razvoja ispituju se biokompatibilnost i utjecaj sustava na permeabilnost lijeka na in vitro modelima. Cilj rada bio je pripremiti praškaste sustave mikrosfera s uklopljenim natrijevim deksametazon fosfatom (NDF-om) i inertnog nosača (laktoze i manitola) te odrediti osmolalnost disperzije sustava, kao i biokompatibilnost sustava te permeabilnost uklopljenog NDF-a korištenjem Calu-3 staničnog modela nazalnog epitela. Metodom sušenja raspršivanjem uspješno su pripravljene mikrosfere graĎene od pektina i hipromeloze, sa sadrţajem uklopljenog NDF-a od 31,6 ± 1,5 %. Miješanjem mikrosfera s inertnim nosačem (laktozom i manitolom) u omjeru 1:9 (m/m) uspješno su pripravljeni praškasti sustavi. OdreĎena je osmolalnost disperzije sustava nakon suspendiranja u smjesi pročišćene vode i HBSS-Ca2+ (1:1, V/V). Vijabilnost Calu-3 staničnog modela nakon izlaganja ispitivanim sustavima bila je u rasponu od 81,5 ± 5,4 % do 113,8 ± 1,6 %. Manitol je pokazao bolja svojstva kao nosač u odnosu na laktozu. Za praškaste sustave s manitolom zabiljeţeno je reverzibilno smanjenje transepitelnog električnog otpora staničnog monosloja tijekom ispitivanja permeabilnosti te značajno veći prividni koeficijent permeabilnosti (Papp) NDF-a kroz Calu-3 stanični monosloj u odnosu na Papp NDF-a iz otopine. Zaključno, pripravljeni praškasti sustav mikrosfera s uklopljenim NDF-om i manitolom kao inertnim nosačem pokazao je potencijal za nazalnu primjenu prema utvrĎenoj biokompatibilnosti i permeabilnosti lijeka in vitro.
Abstract (english)
Nasal drug administration is being examined as a direct delivery path to the central nervous system. The development of new powder drug delivery systems improves the effectiveness of such applications. Biompatibility and impact of system on drug permeability are evaluated early in the development using in vitro models. The aim of this study was to prepare powder systems of microspheres with entrapped sodium dexamethasone phosphate (NDF) and inert carrier (lactose and mannitol), and to determine the osmolality of the dispersion of the systems, their biocompatibility and permeability of the incorporated NDF using a Calu-3 cell model of the nasal epithelium. Microspheres made of pectin and hypromellose with a drug loading of 31.6 ± 1.5 %, were successfully prepared by spray-drying. Powder systems were successfully prepared by mixing microspheres with lactose and mannitol in ratio of 1:9 (w/w). The osmolality of the dispersion of powder systems was determined after suspending in a mixture of purified water and HBSS-Ca2+ (1:1, V/V). Calu-3 cell viability after exposure to the systems ranged from 81.5 ± 5.4 % to 113.8 ± 1.6 %. Mannitol showed better properties as the carrier compared to lactose. For systems with mannitol, a reversible decrease in transepithelial electrical resistance of the cell monolayer and significantly higher apparent permeability coefficient (Papp) of NDF across the Calu-3 monolayer in comparison to Papp of NDF in solution were observed. In conclusion, prepared powder system composed of NDF loaded microspheres and mannitol showed potential for nasal administration considering determined in vitro biocompatibility and drug permeability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:102402
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-05-26 07:54:35