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master's thesis
Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:192724
Šiniković, Miriam
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical botany

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Šiniković, M. (2022). Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:192724

Šiniković, Miriam. "Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:192724

Šiniković, Miriam. "Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:192724

Šiniković, M. (2022). 'Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:192724

Šiniković M. Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:163:192724

M. Šiniković, "Određivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:192724

Metadata
TitleOdređivanje fitotoksičnosti i genotoksičnosti bisfenola A na luku (Allium cepa L.)
Title (english)Determination of phytotoxicity and genotoxicity of bisphenol A on onion (Allium cepa L.)
AuthorMiriam Šiniković
MentorAna-Marija Domijan (mentor)
MentorGoran Gajski (mentor)
Committee memberAna-Marija Domijan (predsjednik povjerenstva)
Committee memberGoran Gajski (član povjerenstva)
Committee memberLovorka Vujić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical botany)
Zagreb
Defense date and country2022-06-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Bisfenol A (BPA) je organski spoj koji se koristi u proizvodnji epoksidnih smola i polikarbonata koji su glavne komponente predmeta u svakodnevnoj upotrebi. Dosadašnja istraživanja su dokazala njegov toksični učinak na okoliš i ljudsko zdravlje pa je glavni cilj ovog istraživanja bio ispitati fitotoksični i genotoksični učinak BPA na meristemske stanice korjenčića luka (Allium cepa L). Allium test ima niz prednosti u istraživanju kao što su jednostavnost rukovanja, veličina i broj kromosoma koji su praktični za analizu kromosomskih aberacija te povoljna cijena. Nakon naklijavanja (dva dana) korjenčići luka (n = 10) bili su izloženi ili BPA (u koncentracijskom rasponu 1-50 mg/L) ili destiliranoj vodi (kontrola) kroz tri dana. Nakon tri dana, pregledana je morfologija korjenčića, izmjerena duljina korjenčića te su pripremljeni mikroskopski preparati meristemskih stanica korjenčića luka u kojima je praćena pojava kromosomskih oštećenja i mikronukleusa. Dobiveni rezultati statistički su obrađeni t-testom (Excel, MS Office), a razina značajnosti postavljena je na p < 0,05. Trodnevni tretman s BPA doveo je do smanjenja produžnog rasta korjenčića te je već nakon tretmana s najnižom ispitivanom koncentracijom (1 mg/L) zabilježena značajno manja duljina korjenčića u odnosu na kontrolu (4,07 ± 0,33 cm vs. 4,61 ± 0,38 cm; p < 0,05). Također, trodnevni tretman s BPA utjecao je i na izgled korjenčića te su s porastom koncentracije BPA korjenčići postali mekši, nepravilni i uvinuti u odnosu na kontrolu. Mikroskopska analiza meristemskih stanica korjenčića luka pokazala je da je trodnevni tretman s BPA doveo do pojave kromosomski oštećenja. S porastom koncentracije BPA povećala se učestalost pojave kromosomskih oštećenja kao što su gubitak kromosoma, lomovi kromosoma i anafazni mostovi. U interfaznim jezgrama meristemskih stanica luka nakon trodnevnog tretmana s BPA zabilježena je pojava mikronukleusa i jezgrinih pupova te je njihova učestalost rasla s povećanjem koncentracije BPA. Rezultati ovog istraživanja pokazuju da BPA ima fitotoksični i genotoksični, ali i potencijalno mutageni učinak na korjenčiće luka.
Abstract (english)
Bisphenol A (BPA) is an organic compound used for production of epoxy resins and polycarbonates that are main components of items used in everyday life. Investigations conducted by now demonstrated its toxicity. Therefore, the aim of this study was to test phytotoxicity and genotoxicity of BPA on meristem cells of onion (Allium cepa L.) roots. The Allium test has number of advantages such as simple handling, size and number of chromosomes which make analysis of chromosome aberrations easy, as well as affordable price. After two-day germination, roots of onion bulbs (n = 10) were exposed to either BPA (in the concentration range of 1-50 mg/L) or distilled water (control) for three days. After three days, the morphology of the roots was examined, and the length of the roots was measured. Additionally, microscopic slides of meristem cells of the onion roots were prepared, in which chromosome aberrations and micronucleus were monitored. The obtained results were statistically analyzed by a t-test (Excel, MS Office), and the level of significance is set to p < 0.05. Three-day treatment with BPA reduced root length and the lowest BPA concentration (1 mg/L) significantly reduced root length compared to the control (4.07 ± 0.33 cm vs. 4.61 ± 0.38 cm; p < 0.05). Additionally, a three-day treatment with BPA had effect on the roots’ appearance; with an increase in the concentration of BPA the roots became softer, irregular, and twisted in comparison to the control. Analysis of onion roots meristem cells revealed that a three-day treatment with BPA led to the chromosome aberrations. The frequency of chromosome damage such as loss of chromosome, chromosome-breaks and anaphase bridges increased with the increase of BPA concentration. In meristem cells in the interphase treatment with BPA induce formation of micronucleus and nuclear buds, and their frequency increased with an increase in BPA concentration. The results of this study demonstrate that BPA has phytotoxic and genotoxic, but also potentially mutagenic effect on onion roots cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:192724
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-07-11 13:49:00