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master's thesis
Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:625243
Horvat, Jasminka
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Horvat, J. (2015). Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:625243

Horvat, Jasminka. "Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:625243

Horvat, Jasminka. "Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:625243

Horvat, J. (2015). 'Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:625243

Horvat J. Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:163:625243

J. Horvat, "Potencijalna primjena mikroRNA u dijagnostici i liječenju bolesti", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:625243

Metadata
TitlePotencijalna primjena mikroRNA u dijagnostici i liječenju bolesti
Title (english)Potential application of microRNA in disease diagnostics and treatment
AuthorJasminka Horvat
MentorSanja Dabelić (mentor)
Committee memberSanja Dabelić
Committee memberSandra Šupraha Goreta
Committee memberTin Weitner
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2015-06-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
miRNA su kratke (18-28 nukleotida), evolucijski visokoočuvane, nekodirajuće RNA molekule koje imaju specifičnu ulogu u regulaciji genske ekspresije. Geni za miRNA se nalaze na kromosomima u jezgri, i na temelju smještaja u genomu dijele se na intergenske i intragenske. Smatra se da je oko 30% protein-kodirajućih gena regulirano pomoću miRNA. Zrela miRNA nastaje djelovanjem brojnih enzima kroz nekoliko koraka, a djeluje u kompleksu koji se naziva miRISC. miRNA se veže na temelju komplementarnosti slijeda „seed“ na 3'-UTR ciljne mRNA, što dovodi do inhibicije translacije ili razgradnje mRNA. Razvijene su brojne metode za izolaciju, detekciju i kvantifikaciju miRNA. Ekspresija miRNA je vrlo precizno regulirana i dinamična u prostoru i vremenu. AntimiRNA i miRNA mimetici su terapeutici temeljeni na miRNA kojima je cilj redukcija ili zamjena miRNA. Cirkulirajuće miRNA su miRNA koje se nalaze u tjelesnim tekućinama i zbog svoje izvanredne stabilnosti mogu se koristiti kao biomarkeri u dijagnostici i prognostici. Promijenjena ekspresija miRNA prisutna je kod svih vrsta tumora. Čimbenici okoliša kojima je plod izložen tijekom unutarmateričnog razvoja uzrokuju promjene u ekspresijskim profilima miRNA koje mogu rezultirati različitim zdravstvenim ishodima kasnije u životu.
Abstract (english)
miRNAs are small (18-28 nt), highly conserved, non-coding RNAs which play specific role in the regulation of gene expression. miRNA genes are located on chromosomes in nucleus, and on the basis of genomic arrangement, they can be classified as intergenic and intragenic. It is estimated that about 30% of protein-coding genes are miRNA-controlled. Mature miRNA is generated by the action of many enzymes through several steps, and acts in complex named miRISC. miRNA binds by its seed sequence to the complementary 3'-UTR of its target mRNA, which results in mRNA degradation or inhibition of translation. Many methods for miRNA isolation, detection and quantification are developed. miRNA expression is highly regulated and dynamic process. AntimiRNAs and miRNAs mimics are sintetic therapeutics based on miRNA with aim to reduce or replace miRNA, respectively. Circulating miRNAs are miRNAs with remarkable stability, which are located in body fluids and can be used as biomarkers for diagnostic and prognostic purposes. Aberrant expressions of miRNAs have been found in all types of tumors. Environmental factors which influence fetus during in-utero development induce changes in miRNA expression profiles which can lead to different health outcomes that manifest later in life.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:625243
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-01-15 10:42:15