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master's thesis
Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:780086
Miholić, Ivana
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmacognosy

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Miholić, I. (2022). Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:780086

Miholić, Ivana. "Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:780086

Miholić, Ivana. "Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:780086

Miholić, I. (2022). 'Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 03 September 2024, https://urn.nsk.hr/urn:nbn:hr:163:780086

Miholić I. Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2024 September 03] Available at: https://urn.nsk.hr/urn:nbn:hr:163:780086

I. Miholić, "Fitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:780086

Metadata
TitleFitokemijski sastav i antioksidativno djelovanje ploda drijena (Cornus mas L.)
Title (english)Phytochemical composition and antioxidant activity of cornelian cherry fruit (Cornus mas L.)
AuthorIvana Miholić
MentorBiljana Blažeković (mentor)
Committee memberBiljana Blažeković (predsjednik povjerenstva)
Committee memberHrvoje Rimac (član povjerenstva)
Committee memberMaja Bival Štefan (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmacognosy)
Zagreb
Defense date and country2022-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
U okviru ovog diplomskog rada istraženi su fitokemijski sastav i antioksidacijska svojstva ploda vrste Cornus mas. Metodom tankoslojne kromatografije u drogi je dokazana prisutnost fenolnih kiselina i antocijana. Spektrofotometrijskim metodama određeno je da ekstrakt drijenovog ploda sadrži 0,32 % fenolnih kiselina, 0,06 % antocijana, 0,21 % trjeslovina te 0,03% flavonoida. Antioksidacijski potencijal ekstrakta drijenovog ploda vrednovan je na temelju sposobnosti hvatanja slobodnih radikala i uspoređen s aktivnošću referentnih antioksidansa (askorbinska kiselina i kvercetin). Utvrđeno je da etanolno-vodeni ekstrakt droge posjeduje sposobnost hvatanja slobodnih DPPH radikala te ostvaruje 50%-tnu učinkovitost pri koncentraciji 76,14 μg/mL. Za razliku od čistih antioksidansa, ispitivani ekstrakt drijenovog ploda nije pokazao značajnu sposobnost hvatanja NO radikala. Dobiveni rezultati doprinose znanstvenim spoznajama o bioaktivnim sastavnicama i antioksidacijskom potencijalu drijena koji raste u Hrvatskoj.
Abstract (english)
In this work, phytochemical composition and antioxidant properties of Cornus mas fruit were studied. Thin-layer chromatography revealed the presence of phenolic acids and anthocyanins in the studied fruit. It was determined by spectrophotometric methods that the cornelian cherry fruit extract contains 0.32% phenolic acids, 0.06% anthocyanins, 0.21 % tannins and 0.03% flavonoids. The antioxidant potential of the extract was evaluated based on the ability to scavenge free radicals and was compared with the activity of reference antioxidants (ascorbic acid and quercetin). It was established that the cornelian cherry fruit extract posses the ability to scavenge DPPH free radicals and achieves 50% efficiency at concentration 7 6.14 μg/mL. In contrast to reference antioxidants, the investigated plant extract did not show significant ability to scavenge NO radicals. The obtained results contribute to scientific knowledge on the bioactive compounds and antioxidant potential of cornelian cherry fruit originating from Croatia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:780086
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-07-21
Terms of useLicence In copyright icon
Created on2022-08-26 11:12:02