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master's thesis
Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:840166
Beljan, Anamarija
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Beljan, A. (2022). Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics) (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:840166

Beljan, Anamarija. "Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:840166

Beljan, Anamarija. "Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:840166

Beljan, A. (2022). 'Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 02 May 2024, https://urn.nsk.hr/urn:nbn:hr:163:840166

Beljan A. Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2024 May 02] Available at: https://urn.nsk.hr/urn:nbn:hr:163:840166

A. Beljan, "Kvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:840166

Metadata
TitleKvantitativna analiza fekalnog kalprotektina novom kemiluminiscentnom metodom na analizatoru Bio-Flash (Inova Diagnostics)
Title (english)Quantitative analysis of faecal calprotectin using a new chemiluminescent method for the Bio-Flash analyzer (Inova Diagnostics)
AuthorAnamarija Beljan
MentorDonatella Verbanac (mentor)
MentorAndrea Tešija Kuna (mentor)
Committee memberDonatella Verbanac (predsjednik povjerenstva)
Committee memberAndrea Tešija Kuna (član povjerenstva)
Committee memberLidija Bach-Rojecky (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2022-09-05, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Fekalni kalprotektin prepoznat je kao specifičan i vrlo osjetljiv biomarker upale crijevne sluznice koji omogućuje razlikovanje upalnih bolesti crijeva od sindroma iritabilnog crijeva. Dijagnostički testovi pojedinih proizvođača temeljeni su na različitim imunokemijskim metodama te još uvijek ne postoji standardiziran postupak ekstrakcije uzorka, pohrane i analize što dovodi do velike varijacije u dobivenim rezultatima i otežava njihovu međusobnu usporedbu. U ovome radu opisano je provođenje postupka verifikacije kvantitativne kemiluminiscentne metode QUANTA Flash® Calprotectin (INOVA Diagnostics, San Diego, CA, USA) za određivanje koncentracije fekalnog kalprotektina u uzorku stolice na analizatoru Bio-Flash (Biokit S.A.©, Španjolska) i usporedbe s postojećim dijagnostičkim testom u rutinskoj primjeni Bühlmann fCAL® turbo (Bühlmann, Schönenbuch, Switzerland) na analizatoru Abbott Architect c8000 (Abbott S.r.l., Roma, Italy) u KBC-u Sestre Milosrdnice. Mjerenja su vršena na 59 ostatnih uzoraka stolica pacijenata kod kojih je zatraženo određivanje fekalnog kalprotektina. Nepreciznost ispitivane metode zadovoljila je zadane kriterije što ju čini prihvatljivom za kliničku primjenu. Unatoč statistički značajnom odstupanju vrijednosti dobivenih ispitivanom i postojećom metodom, podudaranje u kategorizaciji rezultata u dvije interpretativne kategorije pokazalo se snažnim. Zaključeno je da se ispitivana metoda može koristiti u rutinskom radu, ali je preporučljivo za istog pacijenta koncentraciju fekalnog kalprotektina uvijek mjeriti istim testom kako bi rezultati bili međusobno usporedivi jer rezultati dobiveni različitim imunokemijskim metodama nisu međusobno usporedivi i zamjenjivi.
Abstract (english)
Fecal calprotectin was recognized as a specific and sensitive biomarker of intestinal inflammation, making it easier to differentiate inflammatory bowel diseases from irritable bowel syndrome. Numerous existing assays based on different immunochemical methods, along with the lack of standardization of sample extraction, storage, and analysis, have led to significant variation in assays results and exacerbated assay comparability. This thesis describes the verification process of quantitative chemiluminescent method QUANTA Flash® Calprotectin assay (INOVA Diagnostics, San Diego, CA, USA) on analyzer Bio-Flash (Biokit S.A.ã, Spain) and comparison to Bühlmann fCAL® turbo assay (Bühlmann, Schönenbuch, Switzerland) on Abbott Architect c8000 (Abbott S.r.l., Roma, Italy) analyzer which is routinely used within the Department of Clinical Chemistry, University Hospital Centre ''Sestre Milosrdnice'', Zagreb. Samples used for measurement were obtained from remainders of 59 stool samples of patients for whom the clinicians requested the analysis of fecal calprotectin. Assay imprecision met the set criteria, which makes it acceptable for clinical use. Despite statistically significant bias of obtained values, the classification of results of both methods into two categories (positive and negative) proved that the agreement between the two methods is reliable. In conclusion, QUANTA Flash® Calprotectin assay can be used routinely. For follow-up of the same patient, it is advisable always to use the same diagnostic test since the results obtained by different methods are not comparable or interchangeable.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:840166
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-13 12:22:57