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master's thesis
Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:458391
Katavić, Matea
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmacognosy

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Katavić, M. (2022). Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L. (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:458391

Katavić, Matea. "Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:458391

Katavić, Matea. "Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:458391

Katavić, M. (2022). 'Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 14 March 2025, https://urn.nsk.hr/urn:nbn:hr:163:458391

Katavić M. Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2025 March 14] Available at: https://urn.nsk.hr/urn:nbn:hr:163:458391

M. Katavić, "Ultrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:458391

Metadata
TitleUltrazvučna ekstrakcija polifenolnih spojeva iz vrste Plantago major L.
Title (english)Ultrasound-assisted extraction of polyphenolics of Plantago major L.
AuthorMatea Katavić
MentorMarijana Zovko Končić (mentor)
Committee memberMarijana Zovko Končić (predsjednik povjerenstva)
Committee memberJasna Jablan (član povjerenstva)
Committee memberMarijan Marijan (član povjerenstva)
MBZ: 335524
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmacognosy)
Zagreb
Defense date and country2022-11-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Veliki ili širokolisni trputac (Plantago major L., Plantaginaceae) je široko rasprostranjena biljka, koja je poznata u narodnoj medicini već dugi niz godina. Sadrži brojne biološki aktivne spojeve, a to su: flavonoidi, alkaloidi, terpenoidi, iridoidi, vitamini, polisaharidi, masne i fenolne kiseline. Zahvaljujući svom kemijskom sastavu posjedu brojne biološke učinke. Primjerice, pomaže u zacjeljivanju rana, koristan je kod dijareje, pomaže u prevenciji virusnih i bakterijskih infekcija, te... More snižava razinu glukoze i kolesterola u krvi. Polifenoli djeluju protuupalno, antioksidacijski, te smanjuju rizik od nekih kroničnih bolesti, npr. kardiovaskularnih. U ovom radu je za ekstrakciju polifenola iz P.major korištena brza i učinkovita metoda ekstrakcije, ultrazvučna ekstrakcija. Spektrofotometrijski je određena količina ukupnih polifenola, flavonoida i fenolnih kiselina. Koncentracija verbaskozida određena je korištenjem HPLC-a. Usporedbom koncentracija spojeva i uvjeta pripreme ekstrakta ustanovilo se koji su parametri uvjetovali najefikasniju ekstrakciju pojedinih polifenola. Najveći sadržaj ukupnih polifenola imao je ekstrakt koji sadrži 70 % GL, 5 % mliječne kiseline, a ekstrahirao se 30 min pri temperaturi od 70°C i snazi ultrazvuka od 144 W. Flavonoidi su se najbolje ekstrahirali sa 70 % GL, bez mliječne kiseline, a ekstrakcija je trajala 30 min pri 70 °C i snazi ultrazvuka od 720 W. Fenolnim kiselinama je odgovarao medij s 10 % GL i 0,3 mmol HP-β- CD, a ekstrakcija je trajala 30 min pri 20 °C i snazi ultrazvuka od 144 W. Ekstrakcija verbaskozida je najučinkovitije provedena s 10 % GL, 5% mliječne kiseline i 0,3 mmol HP-β-CD, a ekstrakcijsko vrijeme je bilo 10 min na 20 °C i pri snazi ultrazvuka od 144 W. Potrebno je statistički obraditi rezultate kako bi se preciznije odredilo koliko pojedini parametri utječu na ekstrakciju svih navedenih polifenolnih sastavnica. Less
Abstract (english)
Greater or broadleaf plantain (Plantago major L., Plantaginaceae) is a widespread plant, known in folk medicine for many years. It contains numerous biologically active compounds including flavonoids, alkaloids, terpenoids, iridoids, vitamins, polysaccharides, fatty and phenolic acids. Due to its chemical constituents, it has numerous biological effects. For example, it promotes wound healing, prevents viral and bacterial infections, it is useful in treatment of diarrhea, and lowers the... More level of glucose and cholesterol in the blood. Polyphenols act as antioxidants, anti-inflammatory and reduce the risk of some chronic diseases, eg cardiovascular. In this work, a fast and efficient extraction method, ultrasonic extraction, was used for the extraction of polyphenols from P. major. The amount of total polyphenols, flavonoids and phenolic acids was determined spectrophotometrically. Verbascoside concentration was determined using HPLC. By comparing the concentrations of the target compounds and the conditions in which extracts were prepared, the parameters necesary for the most efficient extraction of individual polyphenols were established. The extract with the highest content of total polyphenols was prepared using 70 % GL, 5 % lactic acid and was extracted for 30 min at a temperature of 70 °C with an ultrasound power of 144 W. Flavonoids were best extracted using 70 % GL, without lactic acid, and the extraction lasted 30 min at 70 °C with an ultrasound power of 720 W. The most suitable medium for phenolic acid was with 10 % GL, 0.3 mmol HP-β- CD, and the extraction lasted 30 min at 20 °C with an ultrasound power of 144 W. The extract with 10 % GL, 5% lactic acid, 0.3 mmol HP-β-CD showed the highest verbascoside extraction, and the extraction time was 10 min at 20 °C and with an ultrasound power of 144 W. It is still necessary to statistically process the results in order to precisely determine how certain parameters affect the extraction of all the mentioned polyphenol components. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:458391
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-11-29
Terms of useLicence In copyright icon
Created on2022-12-01 12:18:09
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