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master's thesis
Uloga polimorfizama gena CYP2J2 u kliničkoj medicini
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. urn:nbn:hr:163:139400
Jurič, Magdalena
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Jurič, M. (2023). Uloga polimorfizama gena CYP2J2 u kliničkoj medicini (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:139400

Jurič, Magdalena. "Uloga polimorfizama gena CYP2J2 u kliničkoj medicini." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. https://urn.nsk.hr/urn:nbn:hr:163:139400

Jurič, Magdalena. "Uloga polimorfizama gena CYP2J2 u kliničkoj medicini." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. https://urn.nsk.hr/urn:nbn:hr:163:139400

Jurič, M. (2023). 'Uloga polimorfizama gena CYP2J2 u kliničkoj medicini', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:139400

Jurič M. Uloga polimorfizama gena CYP2J2 u kliničkoj medicini [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2023 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:163:139400

M. Jurič, "Uloga polimorfizama gena CYP2J2 u kliničkoj medicini", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:163:139400

Metadata
TitleUloga polimorfizama gena CYP2J2 u kliničkoj medicini
Title (english)The role of CYP2J2 gene polymorphisms in clinical medicine
AuthorMagdalena Jurič
MentorDunja Rogić (mentor)
MentorLIVIJA ŠIMIČEVIĆ (mentor)
Committee memberDunja Rogić (predsjednik povjerenstva)
Committee memberLIVIJA ŠIMIČEVIĆ (član povjerenstva)
Committee memberAnita Somborac Bačura (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2023-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Enzimi citokrom P450 (CYP) imaju ključnu ulogu u stvaranju epoksieikozatrienoičnih kiselina (EET), metabolita arahidonske kiseline koji ostvaruju različite zaštitne i protuupalne učinke, prvenstveno na kardiovaskularni sustav. One potiču vaskularnu relaksaciju, angiogenezu i fibrinolizu, djeluju protuupalno te moduliraju aktivnost ionskih kanala. CYP2J2 je glavni enzim odgovoran za epoksidaciju arahidonske kiseline u EET u srcu i koronarnim arterijama. Polimorfizam CYP2J2 *7 u promotorskoj regiji gena smanjuje njegovu ekspresiju za 48 % što rezultira smanjenom koncentracijom EET, disregulacijom proupalnih i profibrotičkih čimbenika te promijenjenom aktivnosti ionskih kanala. Kako su upravo ti mehanizmi uključeni u nastanak fibrilacije atrija, hipoteza ove studije jest kako postoji povezanost polimorfizma CYP2J2 *7 i fibrilacije atrija. Budući da CYP2J2 nije eksprimiran u venama te ne doprinosi patogenezi venske tromboze, genotipizirano je 179 ispitanika s fibrilacijom atrija te 102 ispitanika s trombozom. Učestalost alela CYP2J2 *7 u ispitivanoj populaciji bila je 5,51 %. Zbog istih čimbenika rizika za obje bolesti, nije bilo značajne razlike u učestalosti dislipidemije, hipertenzije i šećerne bolesti, ali su se ispitanici razlikovali s obzirom na dob i spol. Regresijsko analizom utvrđeno je kako prisutnost barem jednog varijantnog alela *7 povećava omjer vjerojatnosti za razvoj fibrilacije atrija. Analizirajući podatke prema spolu, nije utvrđena razlika u učestalosti genotipova kod muškaraca dok je kod žena razlika bila statistički značajna (p = 0,047). Pripadajući omjer vjerojatnosti za razvoj fibrilacije atrija kod žena iznosio je 4,010 (95 % CI: 1,127 – 19,041). Sveukupno, rezultati ovog istraživanja upućuju kako polimorfizam CYP2J2 *7 može pridonijeti povećanom riziku za razvoj fibrilacije atrija u hrvatskoj populaciji, osobito u žena.
Abstract (english)
Cytochrome P450 (CYP) enzymes play a crucial role in the formation of epoxyeicosatrienoic acids (EETs), metabolites of arachidonic acid which have various protective and anti-inflammatory effects, particularly on the cardiovascular system. CYP2J2 is the key enzyme responsible for epoxidation of arachidonic acid into EETs in the heart and coronary arteries. The presence of the CYP2J2 *7 polymorphism in the gene's promoter region reduces its expression by 48% which, consequently, leads to decreased EET concentrations, dysregulation of proinflammatory and profibrotic factors, and altered ion channel activity. As these mechanisms are involved in the development of atrial fibrillation, the hypothesis of this study is that there is an association between CYP2J2 *7 polymorphism and atrial fibrillation. Since CYP2J2 is not expressed in veins and has no significant contribution to the pathogenesis of venous thrombosis, 179 subjects with atrial fibrillation and 102 subjects with thrombosis were genotyped. In the studied population the frequency of the CYP2J2 *7 allele was 5.51%. Due to similar risk factors for both diseases, no significant differences between groups were observed in the frequency of dyslipidemia, hypertension, and diabetes. However, differences in age and gender were found. Regression analysis revealed that the occurrence of at least one variant allele *7 increased the odds ratio of atrial fibrillation. The difference in genotype frequency was not determined in men, but a statistically significant difference (p = 0.047) was observed in women. The corresponding odds ratio of atrial fibrillation for women was 4.010 (95 % CI: 1.127 – 19.041). Overall, the results imply that the CYP2J2 *7 polymorphism may contribute to an increased risk of atrial fibrillation in the Croatian population, especially in women.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:139400
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-11-21 14:48:08