Colorectal cancer (CRC) is a significant public health problem due to its high incidence and mortality rate. A key step in the fight against this malignant disease involves early diagnosis. Currently, many studies are focused on testing the method of liquid biopsy as an innovative solution in the field of diagnosis of malignant diseases. With the aim of introducing liquid biopsy into clinical practice, there is a need to determine and validate potential markers for early diagnosis, therapy selection, therapy success monitoring, disease prognosis and early recurrence detection. In this work, the diagnostic potential of the c-MYC gene was examined by comparing the expression of the c-MYC gene in RNA isolated from a tissue of patients with adenoma (pre-CRC) and patients with confirmed CRC. 46 subjects participated in the study, of which 33 subjects had pre-CRC and 13 subjects had confirmed CRC. The results of the analysis did not confirm the potential of the c-MYC gene as a marker for the early detection of CRC, since there was no statistically significant difference between the expression of the c-MYC gene of subjects with pre-CRC and confirmed CRC (p > 0.05). At the same time, the research examined the potential of the c-MYC gene as a marker for early detection of CRC using the liquid biopsy method of 18 subjects with confirmed CRC. Neither the target (c-MYC) nor the reference gene (GAPDH) expression was detected in RNA samples isolated from exosomes, and therefore it was not possible to carry out a further comparison with the results of c-MYC gene expression out of RNA isolated from the tissue of patients with pre-CRC and confirmed CRC. Unsuccessful detection of c-MYC and GAPDH genes may be due to low concentration of RNA isolated from exosomes, degradation of RNA molecules or a high proportion of miRNA molecules out of total isolated RNA.