Title Kemijska karakterizacija i biološki učinci polifenola u vinu
Title (english) Chemical characterization and biological effects of polyphenols in wines
Author Matija Kropek
Mentor Sanda Vladimir-Knežević (mentor)
Committee member Biljana Blažeković (predsjednik povjerenstva)
Committee member Željan Maleš (član povjerenstva)
Committee member Renata Jurišić Grubešić (član povjerenstva)
Granter University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmacognosy) Zagreb
Defense date and country 2023-07-11, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Universal decimal classification (UDC ) 615 - Pharmacology. Therapeutics. Toxicology
Abstract Vina sadrže različite biološki aktivne polifenolne spojeve za koje je utvrđeno da imaju brojne pozitivne učinke na zdravlje.
Polifenolni profil vina često se koristi za procjenu njihove kvalitete i autentičnosti. U okviru doktorskog rada provedena je
usporedna kemijska karakterizacija polifenola u dvadeset i pet komercijalnih crnih i bijelih vina proizvedenih u različitim
hrvatskim vinogradarskim regijama od osam crnih i devet bijelih sorti grožđa. U tu je svrhu razvijena i validirana metoda
visokoučinske tekućinske kromatografije (HPLC-DAD) s direktnim injektiranjem vina. Analizirane su dvadeset i četiri
polifenolne sastavnice vina te razvrstane u pet skupina na temelju njihove strukture: fenolne kiseline, flavan-3-ole, antocijane,
flavonoide i stilbene. Crna vina sadržavala su značajno veće koncentracije polifenola od bijelih vina te je kod njih uočena
velika varijabilnost u sastavu. Specifičnim polifenolnim sastavom i sadržajem posebice su se istaknula vina proizvedena od
autohtonih hrvatskih sorti plavac mali i babić. Primjenom multivarijatne analize ustanovljene su sličnosti i razlike u
polifenolnim profilima hrvatskih monosortnih vina te detektirani važni diferencijatori. Glavni predstavnici polifenolnih
sastavnica koji su bili značajno zastupljeni u istraživanim vinima pokazali su snažno antioksidacijsko djelovanje te sposobnost
inhibicije aktivnosti enzima Src-tirozin-kinaze, α-glukozidaze i acetilkolinesteraze. Provedeno istraživanje pružilo je nove
spoznaje o polifenolnim profilima hrvatskih vina, dajući doprinos njihovoj sortnoj diferencijaciji, autentifikaciji i kontroli
kvalitete. Ustanovljeno je da su analizirana crna vina, prvenstveno ona dobivena iz hrvatskih autohtonih crnih sorti grožđa,
vrijedan izvor polifenola sa snažnim antioksidacijskim svojstvima i sposobnošću inhibicije enzima koji predstavljaju važne
terapijske mete u prevenciji i liječenju nekih kroničnih bolesti povezanih sa starenjem.
Abstract (english) Wines contain various biologically active polyphenolic compounds that are belived to have numerous beneficial effects on
human health. The polyphenolic profile of wines is often used to evaluate their quality and authenticity. In this doctoral thesis, a comparative chemical characterization of polyphenols was performed in twenty-five commercial red and white wines produced in different Croatian wine-growing regions from eight black and nine white grape varieties. For this purpose, a method of high-performance liquid chromatography (HPLC-DAD) with direct injection of wine was developed and validated. Twentyfour polyphenolic components of the wines were studied and classified into five groups based on their structure: phenolic acids, flavan-3-ols, anthocyanins, flavonoids and stilbenes. Black wines contained significantly higher concentrations of polyphenols than white wines, and a great variability in their composition was found. Wines produced from the native Croatian varieties Plavac mali and Babić were characterized by their specific polyphenolic composition and content. Multivariate analysis was used to determine similarities and differences in the polyphenolic profiles of Croatian monovarietal wines and to identify important differentiators. The main representatives of polyphenolic components, which were significantly present in the studied wines, showed strong antioxidant activity and the ability to inhibit the activity of Src tyrosine kinase, α-glucosidase and acetylcholinesterase enzymes. The conducted research provided new insights into the polyphenolic profiles of Croatian wines, thus contributing to their varietal differentiation, authentication and quality control. It was found that the analyzed red wines, especially those obtained from Croatian autochthonous red grape varieties, are a valuable source of polyphenols with strong antioxidant properties and the ability to inhibit enzymes that are important therapeutic targets in the prevention and treatment of some chronic diseases associated with aging.
Keywords
hrvatska vina
polifenoli
HPLC-DAD
PCA
antioksidacijsko djelovanje
inhibicija enzima
Src-tirozinkinaza
α-glukozidaza
acetilkolinesteraza
Keywords (english)
Croatian wines
polyphenols
HPLC-DAD
PCA
antioxidant activity
enzymes inhibition
Src tyrosine kinase
α-glucosidase
acetylcholinesterase
Language croatian
URN:NBN urn:nbn:hr:163:450726
Promotion 2023
Study programme Title: Pharmacy and biochemistry Study programme type: university Study level: postgraduate Academic / professional title: doktor znanosti (doktor znanosti)
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Access conditions Embargoed access Embargo expiration date: 2025-01-11
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Created on 2024-01-23 12:37:24