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master's thesis
Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. urn:nbn:hr:163:029839
Oroz, Helena
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Oroz, H. (2024). Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:029839

Oroz, Helena. "Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:029839

Oroz, Helena. "Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:029839

Oroz, H. (2024). 'Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:029839

Oroz H. Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2024 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:163:029839

H. Oroz, "Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:163:029839

Metadata
TitleUsporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom
Title (english)Comparison of erythrocyte sedimentation rate measurement on automated SRS 100/II analyser and manually by modified Westergren method
AuthorHelena Oroz
MentorMarija Grdić Rajković (mentor)
MentorSandra Margetić (mentor)
Committee memberMarija Grdić Rajković (predsjednik povjerenstva)
Committee memberSandra Šupraha-Goreta (član povjerenstva)
Committee memberSandra Margetić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2024-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Brzina sedimentacije eritrocita rutinska je metoda koja se često koristi u medicinsko-biokemijskim laboratorijima. Westergren metoda referentna je metoda, no danas se često u upotrebi nalaze automatizirane i modificirane metode za određivanje brzine sedimentacije. Svrha ovog ispitivanja usporedba je automatizirane modificirane metode na SRS 100/II analizatoru (Greiner Bio-One, GmbH, Austrija) i ručne modificirane metode na graduiranom stalku. Budući da analiza na SRS 100/II analizatoru i na graduiranom stalku iziskuje nove spremnike, prije usporedbe metoda provedena je tehnička i klinička validacija spremnika VACUETTE® Tube 4NC ESR volumena 1,5 i 2,75 mL s 3,2%-tnim natrijevim citratom. Također, ispitana je i ponovljivost pomoću uzoraka, jednog unutar, jednog iznad referentnog intervala. Utvrđena je primjerenost uvođenja obaju spremnika u rutinski rad, no, s obzirom na to da analizator koristi samo spremnike volumena 1,5 mL, usporedivost je ispitana samo na njima. SRS 100/II posjeduje mogućnost mjerenja u trajanju od 15 i 30 minuta, tako da su uspoređivani rezultati dobiveni i nakon 15 minuta i nakon 30 minuta s rezultatima dobivenim na graduiranom stalku nakon 30 minuta. Statističkom analizom utvrđeno je da za mjerenje u trajanju od 30 minuta ne postoji klinički značajno konstantno odstupanje, dok za mjerenje u trajanju od 15 minuta da postoji klinički značajno konstantno odstupanje, stoga je prihvaćena metoda na SRS 100/II s mjerenjem u trajanju od 30 minuta za uvođenje u rutinski rad. Ispitivanjem ponovljivosti na toj metodi, dobiveni rezultati zadovoljavaju kriterije prihvatljivosti.
Abstract (english)
Erythrocyte sedimentation rate is a routine method commonly used in medical-biochemical laboratories. While the Westergren method is a reference method, today automated and modified methods for measuring the sedimentation rate of erythrocytes are often in use. The purpose of this examination is to compare the automated modified method on SRS 100/II analyzer (Greiner BioOne, GmbH, Austria) and the manual modified method on the graduated stand. Since the analysis on the SRS 100/II analyser and on the graduated stand requires new test tubes, a technical and clinical validation of THE VACUETTE ® tube 4NC ESR test tubes of 1.5 and 2.75 ml with 3.2% sodium citrate was performed prior to the comparison of the methods. Also, repeatability using samples was tested. The appropriateness of introducing both test tubes into routine was established, however, since the analyser only uses 1,5 ml test tubes, comparability was only tested on them. The SRS 100/II has the possibility of measuring for 15 and 30 minutes, so the results obtained after both 15 minutes and 30 minutes were compared with the results obtained on the graduated stand after 30 minutes. A statistical analysis showed that there is no clinically significant constant difference for a 30 minute measurement, while a clinically significant constant difference was found for a 15 minute measurement. Therefore, a 30 minute measurement SRS 100/II method was adopted for routine implementation. By testing repeatability on this method, the results were found to meet the acceptability criteria.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:029839
Study programmeTitle: Medical biochemistry - integrated undergraduate and graduate - university study
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-26 11:10:18