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master's thesis
Slobodna cirkulirajuća DNA u osteoartritisu
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. urn:nbn:hr:163:005480
Ćosić, Klara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Ćosić, K. (2024). Slobodna cirkulirajuća DNA u osteoartritisu (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:005480

Ćosić, Klara. "Slobodna cirkulirajuća DNA u osteoartritisu." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:005480

Ćosić, Klara. "Slobodna cirkulirajuća DNA u osteoartritisu." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:005480

Ćosić, K. (2024). 'Slobodna cirkulirajuća DNA u osteoartritisu', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:005480

Ćosić K. Slobodna cirkulirajuća DNA u osteoartritisu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2024 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:005480

K. Ćosić, "Slobodna cirkulirajuća DNA u osteoartritisu", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:163:005480

Metadata
TitleSlobodna cirkulirajuća DNA u osteoartritisu
Title (english)Circulating free DNA in osteoarthritis
AuthorKlara Ćosić
MentorSandra Šupraha Goreta (mentor)
Committee memberSandra Šupraha-Goreta (predsjednik povjerenstva)
Committee memberLidija Bach-Rojecky (član povjerenstva)
Committee memberNajda Rudman (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2024-08-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Osteoartritis (OA), kronična i degenerativna bolest zglobova, najčešći je oblik artritisa. Često se zanemaruje ozbiljnost ove raširene i kronične bolesti. Razvoj lijeka za modificiranje OA otežan je nedostatkom topivih biomarkera za rano otkrivanje OA. Cilj istraživanja OA biomarkera je identificirati rani OA prije pojave radiografskih znakova i razvoja boli. Slobodna cirkulirajuća DNA (cfDNA) mogla bi poslužiti kao neivazivni biomarker OA. Uspoređene su dvije različite metode izolacije cfDNA i cilj je bio potvrditi koju je od njih bolje koristiti u budućoj genomskoj analizi s obzirom na bolji prinos ili čistoću. Istraživanje izvanstaničnog genomskog materijala također može otkriti više o temeljnoj patofiziologiji OA, stoga postoji potreba za definiranjem biomarkera za otkrivanje i praćenje napredovanja OA bolesti. Minimalno invazivne metode tekuće biopsije kao što su uzorkovanje sinovijalne tekućine i krvi za uzimanje genomskog materijala mogu biti osjetljivije od radiografije u otkrivanju, dijagnozi i praćenju OA u budućnosti.
Abstract (english)
Osteoarthritis (OA), a chronic, debilitating and degenerative disease of the joints, is the most common form of arthritis. The seriousness of this prevalent and chronic disease is often overlooked. Disease modifying OA drug development is hindered by the lack of soluble biomarkers to detect OA early. The objective of OA biomarker research is to identify early OA prior to the appearance of radiographic signs and the development of pain. So, the aim of work was extracellular genomic material that could serve as biomarkers of OA, cell-free DNA (cfDNA). Two different cfDNA isolation methods were compared and the aim was to confirm which one is better to use in future genomic analysis considering better yield or purity. There is an unmet need for soluble biomarkers for detecting and then monitoring OA disease progression. Extracellular genomic material research may also reveal more about the underlying pathophysiology of OA. Minimally invasive liquid biopsies such as synovial fluid and blood sampling of genomic material may be more sensitive over radiography in the detection, diagnosis and monitoring of OA in the future.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:005480
Study programmeTitle: Pharmacy - integrated undergraduate and graduate - university study
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-11 11:35:53