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master's thesis
Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. urn:nbn:hr:163:745741
Hulak, Sara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Hulak, S. (2024). Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:745741

Hulak, Sara. "Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:745741

Hulak, Sara. "Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:163:745741

Hulak, S. (2024). 'Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:745741

Hulak S. Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2024 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:163:745741

S. Hulak, "Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:163:745741

Metadata
TitleIspitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel
Title (english)Biocompatibility study of azithromycin-loaded deformable liposomes incorporated into chitosan and carbopol gel
AuthorSara Hulak
MentorZora Rukavina (mentor)
Committee memberZora Rukavina (predsjednik povjerenstva)
Committee memberLaura Nižić Nodilo (član povjerenstva)
Committee memberEmerik Galić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical technology)
Zagreb
Defense date and country2024-09-05, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Prilikom razvoja hibridnih formulacija liposomi-u-gelu, potrebno je voditi računa ne samo o učinkovitosti i biofarmaceutskim karakteristikama formulacije, već i o biokompatibilnosti s odabranim staničnim kulturama. U sklopu ovog istraživanja deformabilni liposomi s AZT-om (DL) uklopljeni su u 2 različita gela: kitozanski gel (CHG) i karbopolski gel (CAG), s ciljem ispitivanja i usporedbe biokompatibilnosti dobivenih formulacija liposomi-u-gelu in vitro na HaCaT staničnoj liniji. Formulacija DL-CHG je u rasponu koncentracija ≤ 32 μg/ml biokompatibilna s HaCaT stanicama, dok je pri koncentraciji  64 μg/ml vijabilnost stanica bila ˂ 70 % te se može smatrati citotoksičnom za HaCaT stanice. U usporedbi s formulacijom DL-CHG, DL-CAG pokazuje biokompatibilnost u širem rasponu koncentracija (0,25  64 μg/ml), te je pri najvišoj testiranoj koncentraciji vijabilnost tretiranih stanica veća u odnosu na formulaciju DL-CHG pri istoj koncentraciji, zbog čega se CAG može smatrati prikladnijom podlogom za uklapanje DL od CHG. Budući da su se gelovi s uklopljenim neliposomskim lijekom također pokazali citotoksičnima pri visokim koncentracijama AZT-a, čini se da citotoksičnost formulacija DL-u-gelu pri koncentraciji  64 μg/ml proizlazi djelomično iz prisutnosti samog AZT-a u formulaciji, a djelomično je odgovoran sastav liposomske formulacije DL.
Abstract (english)
During the development of hybrid liposome-in-gel formulations, it is essential to consider not only the efficacy and biopharmaceutical characteristics of the formulation but also biocompatibility with selected cell cultures. In this study, AZT-loaded deformable liposomes (DL) were incorporated into two different gels: chitosan gel (CHG) and carbopol gel (CAG), with the aim of testing and comparing the biocompatibility of the obtained liposome-in-gel formulations in vitro on the HaCaT cell line. The DL-CHG formulation was biocompatible with HaCaT cells at a concentration ≤ 32 μg/ml, while at a concentration ≥ 64 μg/ml, cell viability was ˂ 70%, which can be considered cytotoxic. Compared with the DL-CHG formulation, DL-CAG shows biocompatibility over a wider range of concentrations (0.25  64 μg/ml). Moreover, at the highest tested concentration, the viability of treated cells was greater compared to the DL-CHG formulation, making CAG a more suitable vehicle for incorporating DL from a biocompatibility standpoint. Since gels with incorporated nonliposomal AZT also exhibited cytotoxicity at high concentrations, it seems that the cytotoxicity of the DL-in-gel formulations at concentrations ≥ 64 μg/ml arises partly from the presence of AZT itself in the formulation, and partly from the composition of the DL liposome formulation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:745741
Study programmeTitle: Pharmacy - integrated undergraduate and graduate - university study
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-14 12:23:15