Peripheral artery disease is a clinical manifestation of atherosclerosis with significant morbidity and mortality. Inflammation and angiogenesis play an important role in its development, progression and complications. The aim of this thesis was to investigate the association of the catalytic concentrations and genetic polymorphism of paraoxonase (PON1), the catalytic concentrations of platelet activating factor acetylhydrolase (PAF-AH), the concentrations of vascular endothelial growth factor (VEGF), angiopoietin 2 (Ang-2) and its receptor Tie-2, as novel biomarkers of inflammation and angiogenesis with the appearance and degree of stenotic-occlusive changes in the periphearal arteries. Lower catalytic concentrations of PON1 and polymorphisms Q192R and - 108C>T pon1 gene could play a role in the development of peripheral arterial disease. Differences in the frequency of Q and R alleles between patient and control groups may be one of the causes of the reduced catalytic concentrations of PON1, which may contribute to a reduced protective role of HDL-cholesterol and increased risk of early atherosclerosis in the risk group of patients. An increased concentration of Ang-2 and Tie-2 receptor could indicate increased vascular remodeling in response to the presence of risk factors and could be considered new biomarkers of angiogenesis which indicate the presence of peripheral arterial disease. The absence of significant correlation between the concentrations of the biochemical parameters investigated and the angiographic score as a measure of the anatomic extent of atherosclerotic lesions to the peripheral arteries, suggests that other factors are more important in the progression of the disease.