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master's thesis
Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. urn:nbn:hr:163:014838
Marić, Barbara
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Marić, B. (2016). Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:014838

Marić, Barbara. "Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:014838

Marić, Barbara. "Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:014838

Marić, B. (2016). 'Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:014838

Marić B. Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:014838

B. Marić, "Koncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:163:014838

Metadata
TitleKoncentracija malondialdehida i katalitička aktivnost katalaze u uzorcima seruma, EDTA, citratne i heparinske plazme
Title (english)Concentration of malondialdehyde and catalitic activity of catalase in serum, EDTA, citrate and heparin plasma samples
AuthorBarbara Marić
MentorMarija Grdić Rajković (mentor)
MentorAna-Marija Domijan (mentor)
Committee memberMarija Grdić Rajković (predsjednik povjerenstva)
Committee memberAna-Marija Domijan (član povjerenstva)
Committee memberSuzana Inić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2016-09-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Glavni i najistraženiji produkt lipidne peroksidacije je malondialdehid (MDA), čija koncentracija ujedno ukazuje i na oksidativni stres organizma koji za posljedicu ima prekomjerno nakupljanje toksičnih reaktivnih kisikovih spojeva (ROS). Enzim katalaza spada u antioksidcijske enzime koji predstavljaju sekundarnu obranu organizma od reaktivnih kisikovig spojeva (ROS), stoga njegova katalitička aktivnost također ukazuje na stanje oksidativnog sresa. Poznato je da se vrijednost nekih biokemijskih parametara mijenja ovisno o korištenom uzorku (serum ili plazma) ili korištenom antikoagulansu u uzorku plazme, stoga je cilj ovog rada bio utvrditi da li je pri mjerenju parametara oksidacijskog stresa irelevantan odabir uzorka (serum ili plazma) i antikoagulansa, ili postoji li razlika u vrijednostima istih. U ovom radu mjerene su koncentracije oba parametra u uzorku seruma, EDTA plazme, citratne plazme i heparinske plazme.Rezultati ispitivanja pokazuju da se koncentracije MDA statistički značajano razlikuju između citratne plazme i heparinske plazme te između EDTA plazme i citratne plazme. Aktivnost katalaze se statistički značajno razlikuje između seruma i EDTA plazme. Dobiveni rezultati ukazuju da se izmjerena koncentracija MDA i aktivnost katalaze razlikuje između različitih vrsta uzoraka te da je usporedba rezultata moguća samo između istih tipova uzoraka.
Abstract (english)
Malondialdehyde is the main and most researched product of lipid peroxydation. Its concentration indicates oxidative stress in the body that leads to the generation of toxic reactive oxygen species (ROS).Catalase is a very important enzyme in protecting the cell from oxydative damage by Reactive Oxygen Species (ROS), so its level also indicates oxidative stress.It is well known that some biochemisty parameters have different value depending on which sample is been used (serum or plasma) or which anticoagulant agent was used to collect the blood samples. Therefore, the main objective of this work was to establish whether or not there was a difference between the values od MDA and catalase mesured in different samples ( serum, EDTA plasma, citrate plasma or heparin plasma).The results show that there is a statistically significant difference of MDA between citrate plasma and heparin plasma, also in EDTA plasma and citrate plasma. It is also shown that there is a statistically significant difference of catalase between serum and EDTA plasma. The results indicate that the measured concentration of MDA and catalase activity varies between different types of samples and that the comparison of the results is possible only between the same types of samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:014838
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-12-02 10:39:06