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master's thesis
Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. urn:nbn:hr:163:063082
Crnković, Tea
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of organic chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Crnković, T. (2016). Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:063082

Crnković, Tea. "Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:063082

Crnković, Tea. "Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:063082

Crnković, T. (2016). 'Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:063082

Crnković T. Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:063082

T. Crnković, "Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:163:063082

Metadata
TitleInterakcija albumina s nanočesticama srebra različite površinske funkcionalizacije
Title (english)Interaction of albumin and silver nanoparticles with different surface functionalization
AuthorTea Crnković
MentorValerije Vrček (mentor)
Committee memberValerije Vrček (predsjednik povjerenstva)
Committee memberSandra Šupraha Goreta (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of organic chemistry)
Zagreb
Defense date and country2016-06-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
U današnjim predkliničkim testiranjima farmaceutskih molekula, među kojima se nalaze i nanočestice za biomedicinsku primjenu, evaluacija vezanja proteina plazme je najvažniji element kod određivanja efikasnosti, sigurnosti i raspolaganja s tim molekulama. Zbog toga je u ovom istraživanju primijenjen modelni sustav koji se sastojao od goveđeg serumskog albumina (BSA) i 5 vrsta srebrnih nanočestica (AgNP) s različitim površinskim omotačima. Karakterizacija i stabilnost AgNP te njihova interakcija s BSA praćeni su metodama dinamičkog i elektroforetskog raspršenja svjetlosti, transmisijskom elektronskom mikroskopijom, UV-Vis spektrofotometrijom te spektrofluorimetrijom. Otkriveno je da BSA dovodi do koloidne stabilizacije AgNP u fosfatnom puferu neovisno o njihovom kemijskom sastavu, strukturi površine i površinskom naboju. Također su dobivene različite konstante vezanja AgNP i BSA ovisno o površinskoj funkcionalizaciji AgNP i korištenom mediju. Time je potvrđena hipoteza da biomolekule poput BSA mogu pružiti stabilizaciju nanočestica u biološkom okruženju.
Abstract (english)
In today's preclinical testings of pharmaceutical molecules, among which are also present nanoparticlas for biomedical applications, evaluation of plasma protein binding is one of the most important elements in efficency, safety and distribution determination. Therefore, in this thesis a model system of bovine serum albumin (BSA) and 5 types of silver nanoparticles (AgNP) with different surface coatings was used. Characterization and stability of AgNP along with their interaction with BSA were determined by using dynamic and electrophoretic light scattering methods, transmission electron microscopy, UV-Vis spectrophotometry, and spectrofluorimetry. It was discovered that BSA provided colloidal stability for AgNP in phosphate buffer regardless of their chemical composition, surface structure and surface charge. Also, different binding constants of BSA and AgNP were obtained depending on AgNP surface functionalisation and used medium. Thereby, a hypothesis which states that biomolecules like BSA can enhance stabilization of nanoparticles in biological environment was confirmed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:063082
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-12-02 14:18:43