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master's thesis
Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. urn:nbn:hr:163:501568
Petrović, Lidija
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical botany

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Petrović, L. (2016). Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:501568

Petrović, Lidija. "Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:501568

Petrović, Lidija. "Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:501568

Petrović, L. (2016). 'Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:501568

Petrović L. Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2016 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:163:501568

L. Petrović, "Ekspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:163:501568

Metadata
TitleEkspresija izoformi proteina p53 i p73 u stanicama melanoma čovjeka nakon izlaganja gama-zračenju
Title (english)Expression of p53 and p73 protein isoforms in human melanoma cells after exposure to gamma-radiation
AuthorLidija Petrović
MentorAna-Marija Domijan (mentor)
Committee memberAna-Marija Domijan (predsjednik povjerenstva)
Committee memberErim Bešić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical botany)
Zagreb
Defense date and country2016-05-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Obitelj proteina p53 čine strukturno slični transkripcijski faktori s raznolikim biološkim funkcijama koje obuhvaćaju regulaciju staničnog ciklusa, starenje te smrt stanice. Posebno ih zanimljivima čini sposobnost prepisivanja s različitih promotora te široka mogućnost alternativnog prekrajanja što rezultira velikim brojem izoformi. Te su izoforme različite po svojim ulogama u organizmu te međusobno stupaju u interakcije koje rezultiraju aktivacijom ili inaktivacijom pojedinog proteina obitelji p53. U ovom je diplomskom radu proučavana ekspresija proteina p53, koji se naziva čuvarom genoma zbog svoje uloge u sprječavanju tumorigeneze, te proteina p73 koji ima dvostruku ulogu u patogenezi tumora. Stanice melanoma čovjeka, Mel 505, izložene su γ-zračenju doze 5 i 10 Gy s ciljem izazivanja oštećenja DNA i aktivacije proteina obitelji p53. Ekspresija proteina praćena je metodom Western blot analize, a rezultati pokazuju manju promjenu ekspresije proteina p53 te TAp73β izoforme dok je razina ekspresije izoformi ΔNp73α i ΔNp73β značajno rasla u periodu 6-12 sati nakon zračenja. Također, ekspresija tih izoformi veća je pri zračenju dozom od 10 Gy.
Abstract (english)
p53 family of proteins consists of structurally related transcription factors with diverse biological functions including the regulation of cell cycle, aging and cell death. They are particularly interesting because of their ability to use different promoters and wide capacity of alternative splicing which results in large number of isoforms. The isoforms have different biological functions and they interact with each other resulting in activation or inactivation of a particular p53 family protein. In this work we studied the expression of p53 protein, which is known as the guardian of the genome because of its role in preventing tumorigenesis, and protein p73, which has a dual role in the pathogenesis of cancer. Human melanoma cells, Mel 505, were exposed to γirradiation of 5 and 10 Gy in order to induce DNA damage and induction of p53 family proteins. Protein expression was monitored by Western blot analysis, and the results demonstrate a minor change in the expression of p53 isoforms and TAp73β while significant increase of ΔNp73α and ΔNp73β expression was observed in the period of 6-12 hours after radiation. Also, the expression of these isoforms is higherupon irradiation whith the dose of 10 Gy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:501568
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-12-06 14:34:45