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master's thesis
Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:643723
Janković, Monika
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Janković, M. (2015). Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:643723

Janković, Monika. "Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:643723

Janković, Monika. "Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:643723

Janković, M. (2015). 'Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:643723

Janković M. Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:643723

M. Janković, "Razvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:643723

Metadata
TitleRazvoj metode za mjerenje aktivnosti ekto-ATPaze u serumu čovjeka
Title (english)Development of method for measuring ecto-ATPase activity in human blood serum
AuthorMonika Janković
MentorTihana Žanić Grubišić (mentor)
Committee memberTihana Žanić Grubišić
Committee memberAnita Somborac Bačura
Committee memberIvan Pepić
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2015-06-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Ekto-ATPaza je enzim koji pripada skupini E-NTPDaza. Navedeni enzimi su ektoenzimi, što znači da sudjeluju u metabolizmu izvanstaničnih molekula. Ekto-ATPaza sudjeluje u metabolizmu nukleozid difosfata i nukleozid trifosfata, s većim afinitetom prema nukleozid trifosfatima. Metabolizam izvanstaničnih molekula s odgovarajućim enzimima jedan je od koraka u purinergičnoj signalizaciji koja pak posreduje u različitim fiziološkim i patofiziološkim procesima. Stoga se smatra da vrijednost aktivnosti ekto-ATPaze u serumu može biti potencijalni čimbenik u dijagnostici bolesti povezanih s purinergičnom signalizacijom. Aktivnost ekto-ATPaze u serumu mjerena je pomoću Fiske-Subbarow metode, a srednja vrijednost izmerena na 13 kontrolnih uzoraka iznosi 992.3±281.2 nmol/ml/h. Rezultati su pokazali da je za mjerenje aktivnosti najprikladniji volumen uzorka 50 μL, vrijeme inkubacije na 37 °C 60 min te da je potrebno 100 μL 1.12 mol/L TCA za deproteinizaciju uzorka.
Abstract (english)
Ecto-ATPase is an enzyme which belongs to the group of the E-NTPDases. Those enzymes are ectoenzymes, and that they participate in the metabolism of extracellular molecules. Ecto-ATPase participate in metabolism of nucleoside triphosphate and nucleoside diphosphate with higher affinity for the nucleoside triphosphate. The metabolism of extracellular molecules with the appropriate enzymes is one of the steps in purinergic signaling that mediates a variety of physiological and pathophysiological processes. Therefore it is considered that value of ecto-ATPase activity in human serum may be a potential factor in the diagnosis of diseases associated with purinergic signaling. Ecto-ATPase activity in the human serum was measured by Fiske-Subbarow method, and the average value of 13 healthy samples was 992.3±281.2 nmol/ml/h. Results showed that the most suitable sample volume for the measuring enzyme activity is 50 μL, incubation time on 37 °C is 60 min and that 100 μL 1.12 mol/L TCA is required for deproteinization of serum.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:643723
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-01-18 15:46:50