prikaz prve stranice dokumenta Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru
  Download
PDF 1.34 MB
master's thesis
Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. urn:nbn:hr:163:184849
Fijan, Ines
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Fijan, I. (2017). Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:184849

Fijan, Ines. "Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:184849

Fijan, Ines. "Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:184849

Fijan, I. (2017). 'Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:184849

Fijan I. Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:184849

I. Fijan, "Klinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:163:184849

Metadata
TitleKlinička primjena mjerenja hitotriozidaze u plazmi i suhoj kapi krvi na filtar papiru
Title (english)Clinical significance of chitotriosidase in plasma and dried blood spot
AuthorInes Fijan
MentorKsenija Fumić (mentor)
Committee memberKsenija Fumić (predsjednik povjerenstva)
Committee memberSandra Šupraha Goreta (član povjerenstva)
Committee memberMarija Grdić Rajković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2017-01-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Nasljedni metabolički poremećaji su monogenske bolesti, kod kojih se kao posljedica mutacije gena javlja sinteza nefunkcionalnog enzima ili njegova nedostatna sinteza. Zbog toga može doći do nakupljanja supstrata ili jednog od međuprodukata metaboličkog puta. Lizosomske bolesti nakupljanja spadaju u skupinu nasljednih metaboličkih poremećaja. Gaucherova bolest je najčešća lizosomska bolest. Riječ je o sfingolipidozi uzrokovanoj deficijencijom lizosomalnog enzima β- glukocerebrozidaza. Kao rezultat nedostatka β-glukocerebrozidaze dolazi do akumulacije glukozilceramida u lizosomima makrofaga te se tako formiraju Gaucherove stanice u kojima se nakuplja enzim hitotriozidaza. Stoga je hitotriozidaza biomarker koji se može određivati kod Gaucherovih bolesnika. Također smatra se korisnim biomarkerom i u sarkoidozi, multiploj sklerozi te drugim patološkim stanjima. U ovom diplomskom radu mjerila se aktivnost enzim hitotriozidaze u 84 uzorka plazme bolničkih pacijenata te suhe kapi krvi na filtar papiru. Usporedbom rezultata dobivenih u obje vrste uzoraka cilj je bio razviti metodu za određivanje hitotriozidaze u suhoj kapi krvi na filtar papiru jer ona kao uzorak ima veliki broj prednosti u odnosu na plazmu. Nakon uspostavljanja zadovoljavajućih uvjeta mjerenja hitotriozidaze u suhoj kapi krvi na filtar papiru i njezinim mjerenjem spektrofluorimetrijom, dobiveni otkloni fluorescencije pokazali su da korelacija između dobivenih rezultata u suhoj kapi krvi na filter papiru i rezultata u uzorcima plazme s povišenom aktivnosti ne postoji.
Abstract (english)
Hereditary disorders are monogenetic diseases. In this type of disease synthesis of disfunctional enzyme or lack of synthesised enzyme are caused by mutation of a gene. Moreover, those diseases can cause accumulation of certain substrates or intermediates of metabolic pathways. Lysosomal storage disorder is one of the types of this hereditary disorders. Most common one is called Gauche disease. It is a sphingolipidosis caused by the deficiency of glucocerebrosidase. Consequent accumulation of glucosylceramide inside the lysosomes of macrophages results in formation of so called Gaucher cells. Chitotriosidase (CT) is enzyme that can be determined as biomarker in patients with Gaucher disease because it accumulates in macrophages. Moreover, CT is also a useful biomarker in sarcoidosis, multiple sclerosis and samo other conditions. In this work we carried out the analysis of 84 samples of plasma and dried blood spot. In both sample types we measured CT activity.. The goal was to develop a method for determination of CT in dried blood spot, which has many advantages compared to plasma samples. Therefore, after we achieved appropriate conditions for CT determination in dried blood spot we measured CT activity in both samples for each patient using flourescence spectroscopy. Fluorescence readings showed no correlation among dried blood spot samples and plasma samples in which we expected high CT activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:184849
Study programmeTitle: Medical biochemistry
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-14 10:23:28