Warfarin is an oral anticoagulant that is mostly used for the prevention of thromboembolism complications and deep vein thrombosis in patients with atrial fibrillation or venous thromboembolism. Chronic use of this medication requires great commitment and self-control and can greatly affect patient's perception of quality of life. DASS (Duke Anticoagulation Satisfaction Scale) questionnaire is designed to measure treatment satisfaction and quality of life in patients treated with oral anticoagulants. The aim of this study was to measure the satisfaction of patients on anticoagulant therapy with warfarin with the help of DASS questionnaire and to study further the patient's understanding of therapy. A cross-sectional observational study was conducted from March to June 2016 in collaboration with public pharmacies from Zagreb and VelikaGorica. The study involved adult patients who had been on warfarin therapy for more than three months. For each examinee demographic and relevant clinical data were gathered and patients fulfilled the DASS questionnaire. The Ethics Committee from the Faculty of Pharmacy and Biochemistry from University of Zagreb has approved the implementation of this study. The study included 60 patients with an average age of 68.12 (± 11.96) years (range 31-85) that used an average of 5.35 (± 3.13) drugs (range 1-14). The total of 25 questions in DASS questionnaire measured the overall satisfaction in patients on anticoagulants, with possible range of points ranged from 25 to 175 (25 - the highest level of satisfaction; 175 - the lowest level of satisfaction). The average value of the sum of the DASS test sample was 54.55 (± 18.75), indicating a fairly high level of satisfaction. In addition to patient satisfaction, we analysed the connection of DASS results with demographic and general data. Obtained material showed a statistically significant difference in the satisfaction of examinees with respect to sex, concluding that satisfaction with warfarin anticoagulation therapy in men was greater than in women (p <0.008). This research was executed for the first time using the Croatian version of the DASS questionnaire on the Croatian population with the aim to investigate satisfaction and quality of life of patients on warfarin therapy and thus gave preliminary results (obtained on a small sample) on the impact that warfarin therapy has on the quality of patients’ lives.