prikaz prve stranice dokumenta Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke
  Download
PDF 2 MB
master's thesis
Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. urn:nbn:hr:163:163244
Morožin, Teo
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Morožin, T. (2017). Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:163244

Morožin, Teo. "Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:163244

Morožin, Teo. "Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:163244

Morožin, T. (2017). 'Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:163244

Morožin T. Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2017 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:163:163244

T. Morožin, "Optimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:163:163244

Metadata
TitleOptimiranje izolacije slobodne cirkulirajuće DNA u bolesnica oboljelih od raka dojke
Title (english)Optimization of isolation of circulating cell-free DNA in breast cancer
AuthorTeo Morožin
MentorSandra Šupraha Goreta (mentor)
Committee memberSandra Šupraha Goreta (predsjednik povjerenstva)
Committee memberLidija Bach-Rojecky (član povjerenstva)
Committee memberOlga Gornik (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Zagreb
Defense date and country2017-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Rak dojke najčešći je maligni tumor te prvi po mortalitetu od raka u žena u Hrvatskoj. Po svojoj molekularnoj osnovi, postoji 5 podtipova raka dojke: luminalni tip A, luminalni tip B, bazalni tip, HER2 pozitivan i normalni tip. Ovi tipovi se međusobno razlikuju prema prisutnosti i zastupljenosti pojedinih vrsta receptora, a njihovo određivanje je bitno za definiranje terapije te procjenu ishoda liječenja. Trenutne metode koje se koriste pri dijagnostici raka dojke su: citološka dijagnostika, patohistološka dijagnostika, radiološka dijagnostika, magnetska rezonancija dojki, biopsija sentinelnog limfnog čvora. Slobodna cirkulirajuća DNA obuhvaća fragmente nukleinskih kiselina koji cirkuliraju plazmom, serumom ili nekom drugom tjelesnom tekućinom izvan stanice te se uglavnom radi o fragmentima dvolančane DNA veličine do 200 baznih parova. Različita istraživanja su pokazala kako je razina slobodne cirkulirajuće DNA povišena u različitim malignim stanjima pa tako i u raku dojke. Međutim i dalje postoje prepreke za njenu upotrebu kao novog biomarkera u svakodnevnoj kliničkoj praksi. Cilj ovog istraživanja bio je pridonijeti optimiranju metode izolacije slobodne cirkulirajuće DNA u raku dojke. U tu svrhu su korištene različite metode tj. kolone za izolaciju slobodne cirkulirajuće DNA. Istraživanje je pokazalo kako je za izolaciju slobodne cirkulirajuće DNA potrebna upotreba specifičnih kolona kako bi izolacija bila provedena bez tragova genomske DNA koji ometaju detekciju i kvantifikaciju. Također potrebno je oprezno rukovanje s uzorcima, bez opetovanih odmarzavanja i zamrzavanja, s obzirom na to da je slobodna cirkulirajuća DNA manje stabilna od genomske DNA. Nadalje pri usporedbi s kontrolnim uzorcima zdravih dobrovoljaca potreban je njihov pažljiv odabir te dobra diferencijalna dijagnostika jer razina slobodna cirkulirajuća DNA može biti povišena i pri određenim fiziološkim stanjima.
Abstract (english)
Breast cancer is the most common malignancy in women and the first most common cause of cancer death among the female population in Croatia. On molecular basis, there are 5 subtypes of breast cancer: luminal A, luminal B, basal, HER positive and normal type. Those types have different expression level of certain receptors what is important for the choice of therapy and its outcome. Current methods used in breast cancer diagnosis are: cytological diagnostic, histopathological diagnostic, radiological diagnostic, magnet resonance of breasts, the biopsy of sentinel lymph node. Circulating cell free DNA are nucleic acids fragments that circulate in plasma, serum and other bodily fluids outside of cells. Those fragments are composed of double stranded DNA and their size is up to 200 base pairs. Various researches have demonstrated that the level of circulating cell free DNA is elevated in malignancy therefore in breast cancer as well. However, there are still obstacles for its everyday use in clinical practice as a new biomarker. The aim of this research was to contribute to the optimization of isolation of circulating cell free DNA in breast cancer. Different methods and kits for isolations were used. The results showed that for isolation of circulating cell free DNA the specific kits for isolation should be used to eliminate the traces of genomic DNA which may interrupt detection and quantification of cell free DNA. Moreover, the handling with the samples should be very careful, without several freezing and defrosting steps because cell free DNA has the lower level of stability than genomic DNA. Last but not least, considering that level of circulating cell free DNA can be elevated in several physiological conditions, the choice of healthy volunteers should be done very carefully and the differential diagnostic should be done as well.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:163244
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-16 16:08:12